Matsusaka Daniel, Filiault Daniele, Sanchez Diego H, Botto Javier F
IFEVA, UBA, CONICET, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentina.
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, Vienna, Austria.
Front Plant Sci. 2021 Jul 23;12:677728. doi: 10.3389/fpls.2021.677728. eCollection 2021.
shows a wide range of genetic and trait variation among wild accessions. Because of its unparalleled biological and genomic resources, Arabidopsis has a high potential for the identification of genes underlying ecologically important complex traits, thus providing new insights on genome evolution. Previous research suggested that distinct light responses were crucial for Arabidopsis establishment in a peculiar ecological niche of southern Patagonia. The aim of this study was to explore the genetic basis of contrasting light-associated physiological traits that may have mediated the rapid adaptation to this new environment. From a biparental cross between the photomorphogenic contrasting accessions Patagonia (Pat) and Columbia (Col-0), we generated a novel recombinant inbred line (RIL) population, which was entirely next-generation sequenced to achieve ultra-high-density saturating molecular markers resulting in supreme mapping sensitivity. We validated the quality of the RIL population by quantitative trait loci (QTL) mapping for seedling de-etiolation, finding seven QTLs for hypocotyl length in the dark and continuous blue light (Bc), continuous red light (Rc), and continuous far-red light (FRc). The most relevant QTLs, and , were mapped close together to chromosome V; the former for Rc and Rc/dark, and the latter for Bc, FRc, and dark treatments. The additive effects of both QTLs were confirmed by independent heterogeneous inbred families (HIFs), and we explored and as potential candidate genes for and QTLs, respectively. We conclude that the Pat × Col-0 RIL population is a valuable novel genetic resource to explore other adaptive traits in Arabidopsis.
研究表明野生种质间存在广泛的遗传和性状变异。由于拟南芥拥有无与伦比的生物学和基因组资源，它在鉴定具有重要生态意义的复杂性状潜在基因方面具有很高的潜力，从而为基因组进化提供新的见解。先前的研究表明，不同的光反应对于拟南芥在巴塔哥尼亚南部特殊生态位中的定殖至关重要。本研究的目的是探索可能介导了对这一新环境快速适应的与光相关的生理性状差异的遗传基础。通过光形态建成差异显著的巴塔哥尼亚（Pat）和哥伦比亚（Col-0）两个亲本杂交，我们构建了一个新的重组自交系（RIL）群体，对其进行全基因组测序以获得超高密度的饱和分子标记，从而实现极高的定位灵敏度。我们通过对幼苗去黄化的数量性状基因座（QTL）定位验证了RIL群体的质量，在黑暗以及连续蓝光（Bc）、连续红光（Rc）和连续远红光（FRc）条件下，发现了7个控制下胚轴长度的QTL。最相关的QTL， 和 ，紧密连锁在第五条染色体上；前者与Rc和Rc/黑暗条件相关，后者与Bc、FRc和黑暗处理相关。两个QTL的加性效应通过独立的异质近交家系（HIFs）得到证实，我们分别探索了 和 作为 和QTL的潜在候选基因。我们得出结论，Pat×Col-0 RIL群体是探索拟南芥其他适应性性状的宝贵新遗传资源。
