Ruiz-Diaz María Jimena, Matsusaka Daniel, Cascales Jimena, Sánchez Diego H, Sánchez-Lamas Maximiliano, Cerdán Pablo D, Botto Javier F
IFEVA (CONICET-UBA), Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentina.
Fundación Instituto Leloir, IIBBA-CONICET, Buenos Aires, Argentina.
Front Plant Sci. 2022 Sep 7;13:952214. doi: 10.3389/fpls.2022.952214. eCollection 2022.
shows a wide range of natural genetic variation in light responses. Shade avoidance syndrome is a strategy of major adaptive significance that includes seed germination, elongation of vegetative structures, leaf hyponasty, and acceleration of flowering. Previously, we found that the southernmost Arabidopsis accession, collected in the south of Patagonia (Pat), is hyposensitive to light and displays a reduced response to shade light. This work aimed to explore the genetic basis of the shade avoidance response (SAR) for hypocotyl growth by QTL mapping in a recently developed 162 RIL population between Col-0 and Pat. We mapped four QTL for seedling hypocotyl growth: and QTL in white light, QTL in shade light, and QTL for the SAR. is the strongest candidate gene for QTL. Here we studied the function of two polymorphic indels in the promoter region, a GGGR deletion, and three non-synonymous polymorphisms on the coding region compared with the Col-0 reference genome. To decipher the contribution and relevance of each -Pat polymorphism, we constructed transgenic lines with single or double polymorphisms by using Col-0 as a reference genome. We found that single polymorphisms in the coding region of have discrete functions in seed germination, seedling development, and shade avoidance response. These results suggest distinct functions for each polymorphism to the adjustment of plant development to variable light conditions.
在光反应中表现出广泛的自然遗传变异。避荫综合征是一种具有重要适应性意义的策略，包括种子萌发、营养结构伸长、叶片下弯和开花加速。此前，我们发现从巴塔哥尼亚南部（Pat）收集的最南端拟南芥种质对光不敏感，对遮荫光的反应减弱。这项工作旨在通过在最近构建的Col-0和Pat之间的162个重组自交系群体中进行QTL定位，探索下胚轴生长的避荫反应（SAR）的遗传基础。我们定位了四个与幼苗下胚轴生长相关的QTL：白光下的 和 QTL、遮荫光下的 QTL以及SAR的 QTL。 是 QTL的最强候选基因。在这里，与Col-0参考基因组相比，我们研究了启动子区域两个多态性插入缺失（一个GGGR缺失）以及 编码区三个非同义多态性的功能。为了解析每个 -Pat多态性的贡献和相关性，我们以Col-0为参考基因组构建了具有单多态性或双多态性的转基因系。我们发现 编码区的单多态性在种子萌发、幼苗发育和避荫反应中具有不同的功能。这些结果表明每个 多态性在植物发育对可变光照条件的调节中具有不同的功能。
