Zhu Jianhua, Huang Yan, Zhang Yong, Huang Rongfu, Huang Chunmei
Laboratory of Clinical Immunology, Wuhan No.1 Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Front Genet. 2021 Jul 22;12:655569. doi: 10.3389/fgene.2021.655569. eCollection 2021.
Long non-coding RNAs (lncRNAs) have been reported to play a crucial role in the pathogenesis of numerous cancers. However, the function of lncRNA KCNMB2-AS1 in bladder cancer (BC) remains unclear. In the present study, we aimed to explore the role and underlying mechanisms of KCNMB2-AS1 in bladder cancer progression. We found that lncRNA KCNMB2-AS1 was significantly upregulated both in BC tissues and cell lines, the expression level was highly correlated with pathological TNM stage. Functionally, knockdown of lncRNA KCNMB2-AS1 dramatically inhibited the proliferation, migration, and invasion and of BC cells , and suppressed tumor growth . Mechanistically, lncRNA KCNMB2-AS1 could function as a competitive endogenous RNA (ceRNA) through direct sponging miR-374a-3p, which regulated the expression of S100A10. In conclusion, our results demonstrated that lncRNA KCNMB2-AS1 can promote the progression of bladder cancer through regulation of miR-374a-3p/S100A10.
据报道，长链非编码RNA（lncRNAs）在多种癌症的发病机制中起关键作用。然而，lncRNA KCNMB2-AS1在膀胱癌（BC）中的功能仍不清楚。在本研究中，我们旨在探讨KCNMB2-AS1在膀胱癌进展中的作用及潜在机制。我们发现lncRNA KCNMB2-AS1在BC组织和细胞系中均显著上调，其表达水平与病理TNM分期高度相关。在功能上，敲低lncRNA KCNMB2-AS1可显著抑制BC细胞的增殖、迁移和侵袭，并抑制肿瘤生长。机制上，lncRNA KCNMB2-AS1可通过直接结合miR-374a-3p作为竞争性内源性RNA（ceRNA）发挥作用，从而调控S100A10的表达。总之，我们的结果表明lncRNA KCNMB2-AS1可通过调控miR-374a-3p/S100A10促进膀胱癌进展。
