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长链非编码 RNA KCNMB2-AS1 通过 N6-甲基腺苷修饰稳定促进宫颈癌生长，作为竞争性内源性 RNA。

Long Noncoding RNA KCNMB2-AS1 Stabilized by N-Methyladenosine Modification Promotes Cervical Cancer Growth Through Acting as a Competing Endogenous RNA.

机构信息

Department of Gynaecology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.

出版信息

Cell Transplant. 2020 Jan-Dec;29:963689720964382. doi: 10.1177/0963689720964382.

DOI:10.1177/0963689720964382
PMID:33028109
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7784579/
Abstract

Long noncoding RNA (lncRNA) is emerging as an essential regulator in the development and progression of cancer, including cervical cancer (CC). In this study, we found a CC-related lncRNA, KCNMB2-AS1, which was significantly overexpressed in CC and linked to poor outcomes. Depletion of KCNMB2-AS1 remarkably inhibited CC cell proliferation and induced apoptosis. xenograft models revealed that knockdown of KCNMB2-AS1 evidently delayed tumor growth. Mechanistically, KCNMB2-AS1 was predominantly located in the cytoplasm and served as a competing endogenous RNA to abundantly sponge miR-130b-5p and miR-4294, resulting in the upregulation of IGF2BP3, a well-documented oncogene in CC. Moreover, IGF2BP3 was able to bind KCNMB2-AS1 by three N-methyladenosine (mA) modification sites on KCNMB2-AS1, in which IGF2BP3 acted as an mA "reader" and stabilized KCNMB2-AS1. Thus, KCNMB2-AS1 and IGF2BP3 formed a positive regulatory circuit that enlarged the tumorigenic effect of KCNMB2-AS1 in CC. Together, our data clearly suggest that KCNMB2-AS1 is a novel oncogenic mA-modified lncRNA in CC, targeting KCNMB2-AS1 and its related molecules implicate the therapeutic possibility for CC patients.

摘要

长链非编码 RNA（lncRNA）作为癌症（包括宫颈癌）发生和发展的重要调控因子正在兴起。在这项研究中，我们发现了一个与宫颈癌相关的 lncRNA，KCNMB2-AS1，它在宫颈癌中显著过表达，并与不良预后相关。KCNMB2-AS1 的耗竭显著抑制了宫颈癌细胞的增殖并诱导了细胞凋亡。异种移植模型表明，KCNMB2-AS1 的敲低明显延迟了肿瘤的生长。机制上，KCNMB2-AS1 主要位于细胞质中，作为竞争内源性 RNA 大量吸收 miR-130b-5p 和 miR-4294，导致 IGF2BP3 的上调，IGF2BP3 是宫颈癌中公认的癌基因。此外，IGF2BP3 能够通过 KCNMB2-AS1 上三个 N6-甲基腺苷（mA）修饰位点与 KCNMB2-AS1 结合，其中 IGF2BP3 作为 mA“阅读器”并稳定 KCNMB2-AS1。因此，KCNMB2-AS1 和 IGF2BP3 形成了一个正反馈调节回路，放大了 KCNMB2-AS1 在宫颈癌中的致瘤作用。总之，我们的数据清楚地表明，KCNMB2-AS1 是宫颈癌中一种新型的癌基因 mA 修饰 lncRNA，靶向 KCNMB2-AS1 及其相关分子为宫颈癌患者提供了治疗的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/23e830698ecb/10.1177_0963689720964382-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/1e17f1d5bcbe/10.1177_0963689720964382-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/b33968e16f0f/10.1177_0963689720964382-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/f39c61569177/10.1177_0963689720964382-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/47919c8053e7/10.1177_0963689720964382-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/35161d4fdc23/10.1177_0963689720964382-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/b91e7787981c/10.1177_0963689720964382-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/23e830698ecb/10.1177_0963689720964382-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/1e17f1d5bcbe/10.1177_0963689720964382-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/b33968e16f0f/10.1177_0963689720964382-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/f39c61569177/10.1177_0963689720964382-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/47919c8053e7/10.1177_0963689720964382-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/35161d4fdc23/10.1177_0963689720964382-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/b91e7787981c/10.1177_0963689720964382-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da81/7784579/23e830698ecb/10.1177_0963689720964382-fig7.jpg

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本文引用的文献

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2
Epigenetic Regulation of mA Modifications in Human Cancer.人类癌症中 mA 修饰的表观遗传调控
Mol Ther Nucleic Acids. 2020 Mar 6;19:405-412. doi: 10.1016/j.omtn.2019.11.022. Epub 2019 Nov 29.
3
HLA-F-AS1/miR-330-3p/PFN1 axis promotes colorectal cancer progression.HLA-F-AS1/miR-330-3p/PFN1 轴促进结直肠癌进展。
卵子发生和胎儿发育中的表观遗传调控：对m6A修饰的见解
Front Immunol. 2025 Apr 28;16:1516473. doi: 10.3389/fimmu.2025.1516473. eCollection 2025.
4
Epigenetic regulation in female reproduction: the impact of m6A on maternal-fetal health.女性生殖中的表观遗传调控：m6A对母婴健康的影响。
Cell Death Discov. 2025 Feb 4;11(1):43. doi: 10.1038/s41420-025-02324-z.
5
LIN28A-dependent lncRNA NEAT1 aggravates sepsis-induced acute respiratory distress syndrome through destabilizing ACE2 mRNA by RNA methylation.LIN28A依赖的长链非编码RNA NEAT1通过RNA甲基化使ACE2信使核糖核酸不稳定，加重脓毒症诱导的急性呼吸窘迫综合征。
J Transl Med. 2025 Jan 6;23(1):15. doi: 10.1186/s12967-024-06032-7.
6
Epitranscriptomics and cervical cancer: the emerging role of mA, mC and mA RNA modifications.表观转录组学与宫颈癌：m~A、m~C 和 m~A RNA 修饰的新兴作用。
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7
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9
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Life Sci. 2020 Aug 1;254:117180. doi: 10.1016/j.lfs.2019.117180. Epub 2019 Dec 18.
4
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5
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6
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7
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