Khoubrouypak Zahra, Abbasi Mahdi, Ahmadi Elham, Rafeie Niyousha, Behroozibakhsh Marjan
Department of Operative Dentistry, School of Dentistry, Guilan University of Medical Sciences, Rasht, Guilan, Iran.
Department of Operative Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Int J Dent. 2021 Jul 27;2021:3298515. doi: 10.1155/2021/3298515. eCollection 2021.
Considering the suggested advantages of cold atmospheric plasma (CAP) in increasing the fluoride uptake by the enamel, this study aimed to assess enamel erosion following the application of helium CAP and two types of fluoride varnishes.
The microhardness of 70 bovine enamel specimens was measured using a Vickers hardness tester. The specimens were randomly divided into 7 groups ( = 10): control, CAP (P), resin-containing fluoride varnish (RF), CAP + resin-containing fluoride varnish (PRF), fluoride varnish (F), CAP + fluoride varnish (PF), and erosion (E). The specimens in the control and erosion groups did not receive CAP or fluoride varnish. All specimens underwent erosive challenge 4 times/day using hydrochloric acid and artificial saliva except for the control specimens that remained in distilled water during the course of the study. After 5 days of erosive challenge, microhardness was measured again, and the percentage of microhardness change was calculated. Surface roughness of two specimens in each group was assessed by atomic force microscopy (AFM). Data were analyzed using one-way ANOVA followed by Tamhane's post-hoc test.
The percentage of microhardness change in all groups was significantly higher than that of the control group. All groups showed significantly lower percentage of microhardness change compared with the E group except for the P group; no significant difference was noted in microhardness change of P and E groups. Other experimental groups had no significant difference with each other. Surface roughness was the highest in PRF and the lowest in the F group.
CAP application had no significant effect on increasing the enamel resistance to erosion. However, enamel resistance to erosion increased significantly after fluoride varnish application alone or fluoride varnish application combined with CAP. No significant difference was noted between the two types of varnishes in this regard. CAP increased the surface roughness while fluoride varnish application alone decreased the roughness.
鉴于冷大气等离子体（CAP）在增加牙釉质氟摄取方面的潜在优势，本研究旨在评估应用氦气CAP和两种类型的氟漆后牙釉质的侵蚀情况。
使用维氏硬度测试仪测量70个牛牙釉质标本的显微硬度。标本随机分为7组（每组n = 10）：对照组、CAP组（P）、含树脂氟漆组（RF）、CAP + 含树脂氟漆组（PRF）、氟漆组（F）、CAP + 氟漆组（PF）和侵蚀组（E）。对照组和侵蚀组的标本未接受CAP或氟漆处理。除对照组标本在研究过程中置于蒸馏水中外，所有标本每天使用盐酸和人工唾液进行4次侵蚀挑战。在进行5天的侵蚀挑战后，再次测量显微硬度，并计算显微硬度变化百分比。通过原子力显微镜（AFM）评估每组两个标本的表面粗糙度。数据采用单因素方差分析，随后进行Tamhane事后检验。
所有组的显微硬度变化百分比均显著高于对照组。除P组外，所有组的显微硬度变化百分比均显著低于E组；P组和E组的显微硬度变化无显著差异。其他实验组之间无显著差异。表面粗糙度在PRF组最高，在F组最低。
应用CAP对增加牙釉质抗侵蚀能力无显著影响。然而，单独应用氟漆或氟漆与CAP联合应用后，牙釉质抗侵蚀能力显著增加。在这方面，两种类型的漆之间无显著差异。CAP增加了表面粗糙度，而单独应用氟漆降低了粗糙度。
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