Forge A, Wright A, Davies S J
EM Unit, Institute of Laryngology and Otology, London, U.K.
Hear Res. 1987 Dec 31;31(3):253-65. doi: 10.1016/0378-5955(87)90195-x.
Changes in the stria vascularis following chronic gentamicin treatment have been examined using quantitative methods. Albino guinea pigs were given gentamicin at 100 mg.kg-1.day-1 subcutaneously for 10 days. Comparisons were made of strial tissue from the treated animals sacrificed either 1 h or 4 weeks following the last injection with that from saline-injected controls. Strial width (spiral prominence to Reissner's membrane) and marginal cell (MC) number across the stria were determined from scanning electron micrographs. Strial thickness (endolymphatic surface to spiral ligament) and the volume fractions of the strial components (MCs, intermediate cells (ICs), basal cells (BCs) and capillaries) were derived from thin sections. Qualitative changes to both MCs and ICs were apparent 1 h after the last injection. At four weeks post-treatment, there was a small, but statistically significant, decrease in the number of marginal cells and a highly significant decrease in strial thickness. This was almost entirely due to a highly significant decrease in the volume fraction of MCs (i.e. shrinkage). The volume fraction of ICs was increased but this could be accounted for by MC shrinkage; after allowing for the reduction in strial thickness, the volume occupied by ICs was unchanged. Thus, following chronic gentamicin treatment, the stria is affected but significant progressive and permanent structural effects are confined to the marginal cells.
采用定量方法研究了慢性庆大霉素治疗后血管纹的变化。对白化豚鼠皮下注射庆大霉素，剂量为100mg·kg⁻¹·天⁻¹，持续10天。将末次注射后1小时或4周处死的治疗组动物的纹状组织与注射生理盐水的对照组动物的纹状组织进行比较。通过扫描电子显微镜照片确定纹状宽度（螺旋隆起至Reissner膜）和纹状区域的边缘细胞（MC）数量。从薄切片得出纹状厚度（内淋巴表面至螺旋韧带）以及纹状成分（MC、中间细胞（IC）、基底细胞（BC）和毛细血管）的体积分数。末次注射后1小时，MC和IC均出现明显的定性变化。治疗后四周，边缘细胞数量有小幅但具有统计学意义的减少，纹状厚度有极显著的减少。这几乎完全是由于MC体积分数极显著降低（即萎缩）所致。IC的体积分数增加，但这可由MC萎缩来解释；考虑到纹状厚度的减少后，IC所占体积未变。因此，慢性庆大霉素治疗后，血管纹受到影响，但显著的进行性和永久性结构效应仅限于边缘细胞。
