Mostyn Amanda, Lenihan Marie, O'Sullivan Donnchadh, Woods Sara, O'Hara Maureen, Powell James, Power Lorraine, O'Connell Nuala H, Dunne Colum P
Department of Clinical Microbiology, University Hospital Limerick, Limerick, Ireland.
Graduate Entry Medical School and Centre for Interventions in Infection, Inflammation & Immunity (4i), University of Limerick, Limerick, Ireland.
Infect Prev Pract. 2020 Feb 11;2(2):100042. doi: 10.1016/j.infpip.2020.100042. eCollection 2020 Jun.
Rapid and accurate diagnosis of meningitis/encephalitis (M/E) is essential for successful patient outcomes. The FilmArray® meningitis/encephalitis Panel (MEP) is a multiplexed PCR test for simultaneous, rapid detection of pathogens directly from cerebrospinal fluid (CSF) samples. 94 prospectively collected CSF specimens from patients with clinical suspicion of infective M/E underwent testing for 14 pathogens simultaneously, including , , , and . MEP demonstrated 95% agreement with current PCR methods, resulting in 16 diagnosed cases of M/E. Typically, the FilmArray® MEP results were delivered within approximately one hour, contrasting with current practices taking up to 5.6 days. Given the significant morbidity and mortality associated with delayed diagnosis of central nervous system infections, the FilmArray® MEP is a useful addition to the diagnostic capabilities of a clinical microbiology department.
快速准确地诊断脑膜炎/脑炎（M/E）对于患者获得成功治疗结果至关重要。FilmArray®脑膜炎/脑炎检测板（MEP）是一种多重聚合酶链反应（PCR）检测方法，可直接从脑脊液（CSF）样本中同时快速检测病原体。对94例临床怀疑为感染性M/E患者前瞻性收集的CSF标本进行检测，可同时检测14种病原体，包括[此处原文缺失病原体名称]。MEP与当前PCR方法的一致性为95%，确诊16例M/E病例。通常，FilmArray® MEP检测结果大约在1小时内得出，而目前的检测方法则需要长达5.6天。鉴于中枢神经系统感染延迟诊断会带来显著的发病率和死亡率，FilmArray® MEP是临床微生物科诊断能力的一项有益补充。
