猪组织中氨苯砜磺胺的液相色谱测定法。
Liquid chromatographic determination of sulfamoyldapsone in swine tissues.
作者信息
Endoh Y S, Yamaoka R, Sasaki N
机构信息
Ministry of Agriculture, Forestry and Fisheries, National Veterinary Assay Laboratory, Tokyo, Japan.
出版信息
J Assoc Off Anal Chem. 1987 Nov-Dec;70(6):1031-2.
A liquid chromatographic (LC) method is described for the quantitative determination of sulfamoyldapsone (2-sulfamoyl-4,4'-diaminodiphenyl sulfone) in swine muscle, liver, kidney, and fat. Sulfamoyldapsone was extracted from tissues with acetonitrile saturated with n-hexane. The extract was washed with n-hexane saturated with acetonitrile, concentrated, and cleaned up by alumina column chromatography. Sulfamoyldapsone was separated on an ODS column by using acetonitrile-methanol-water (6 + 18 + 76) and was detected at 292 nm. Overall average recovery of sulfamoyldapsone added to tissues at levels of 0.1 and 0.5 microgram/g was 93.3% +/- 6.0. Detection limit was 0.02 microgram/g in these tissues.
本文描述了一种液相色谱（LC）法，用于定量测定猪肌肉、肝脏、肾脏和脂肪中的氨苯砜磺胺（2-磺胺基-4,4'-二氨基二苯砜）。用正己烷饱和的乙腈从组织中提取氨苯砜磺胺。提取物用乙腈饱和的正己烷洗涤，浓缩，并用氧化铝柱色谱法净化。在ODS柱上，使用乙腈-甲醇-水（6 + 18 + 76）分离氨苯砜磺胺，并在292 nm处进行检测。向组织中添加水平为0.1和0.5微克/克的氨苯砜磺胺，其总体平均回收率为93.3% +/- 6.0。这些组织中的检测限为0.02微克/克。
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