Waggoner T B, Bowman M C
Mobay Corp., Animal Health Division, Shawnee Mission, KS 66201.
J Assoc Off Anal Chem. 1987 Sep-Oct;70(5):813-8.
A spectrofluorometric (SPF) method is described for determination of residues of BAY Vp 2674 in chicken and turkey tissues. The drug is extracted from tissues with dichloromethane-methanol. The organic extract is concentrated to near dryness and cleaned up by a series of partitionings with n-hexane, then dichloromethane against pH 2 buffer and dichloromethane against pH 12 buffer. The drug is partitioned into dichloromethane from pH 7 buffer and concentrated to dryness. The residue is dissolved in pH 3.5 buffer for SPF analysis at 282 nm (excitation) and 445 nm (emission). Recoveries of BAY Vp 2674 added to chicken and turkey tissues at levels of 0.05, 0.1, and 0.2 ppm range from 86 to 92% with a coefficient of variation of 3.4-10.1%. Detection limit is 0.02 ppm. A liquid chromatographic confirmatory procedure is also described, with ultraviolet and fluorescence detection.
本文描述了一种用于测定鸡肉和火鸡组织中BAY Vp 2674残留量的荧光分光光度法（SPF）。用二氯甲烷 - 甲醇从组织中提取该药物。有机提取物浓缩至近干，然后通过一系列用正己烷、pH 2缓冲液的二氯甲烷以及pH 12缓冲液的二氯甲烷进行分配来净化。药物从pH 7缓冲液中分配到二氯甲烷中并浓缩至干。残留物溶解在pH 3.5缓冲液中，用于在282 nm（激发）和445 nm（发射）处进行SPF分析。添加到鸡肉和火鸡组织中的BAY Vp 2674在0.05、0.1和0.2 ppm水平的回收率为86%至92%，变异系数为3.4 - 10.1%。检测限为0.02 ppm。还描述了一种液相色谱确证方法，采用紫外和荧光检测。
