Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.
Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan.
Analyst. 2021 Sep 13;146(18):5584-5591. doi: 10.1039/d1an00973g.
COVID-19 has shown that a highly specific and rapid diagnostic system is a necessity. A spectral imaging-based surface plasmon resonance (SPRi) platform with an integrated microfluidic biosensor to detect oligonucleotide sequences has been proposed to be a promising alternative for infectious diseases due to its safe and straightforward use. Approaches to reduce the DNA probe loading onto gold nanoparticles with various types of polyethylene glycol (PEG) were explored. Here, we demonstrated the stability of functionalised gold nanoparticles with unmodified PEG whilst lowering the probe loading density. The system was evaluated by performing the detection of a mimicking COVID-19 target sequence, single point-mutation sequence and fully mismatch sequence. Highly specific binding of the mimicking COVID-19 target sequence was observed and analysed by the spectral imaging SPR approach. Our work has demonstrated the potential of a controlled probe density using unmodified PEG as an especially promising functionalisation strategy in SPR spectral imaging assays.
COVID-19 表明，高度特异和快速的诊断系统是必要的。一种基于光谱成像的表面等离子体共振（SPRi）平台，结合集成微流控生物传感器，用于检测寡核苷酸序列，由于其安全且简单易用，被认为是传染病的一种有前途的替代方法。已经探索了各种类型的聚乙二醇（PEG）来减少 DNA 探针在金纳米粒子上的加载量。在这里，我们证明了功能化的金纳米粒子在使用未修饰的 PEG 时的稳定性，同时降低了探针的加载密度。通过检测模拟 COVID-19 的目标序列、单点突变序列和完全不匹配序列来评估该系统。通过光谱成像 SPR 方法观察到并分析了模拟 COVID-19 目标序列的高度特异性结合。我们的工作表明，使用未修饰的 PEG 作为控制探针密度的一种有前途的功能化策略，在 SPR 光谱成像分析中具有很大的潜力。
