Lacroix C, Poncet P, Laine G, Guyonnaud C, Ray M, Menager S, Lafont O
Unités de Pharmacocinétique, Centre Hospitalier Général, Le Havre, France.
J Chromatogr. 1987 Nov 27;422:217-25.
The method reported here for determining pyrazinamide and its metabolites (2-pyrazinoic acid, 5-hydroxypyrazinamide, 5-hydroxypyrazinoic acid and pyrazinuric acid) consists of diluting urine or acid deproteinisation of serum followed by chromatography on a cation-exchange column. The column length and the detection system (ultraviolet or fluorimetry) allow for a very good separation of the different compounds; the sensitivity of the method makes it suitable for pharmacokinetic studies.
本文报道的测定吡嗪酰胺及其代谢物（2-吡嗪酸、5-羟基吡嗪酰胺、5-羟基吡嗪酸和吡嗪尿酸）的方法包括稀释尿液或对血清进行酸脱蛋白处理，然后在阳离子交换柱上进行色谱分析。柱长和检测系统（紫外或荧光法）能很好地分离不同化合物；该方法的灵敏度使其适用于药代动力学研究。
