一种定量测定天冬氨酸氨基转移酶同工酶的动力学方法。

A kinetic method for quantification of aspartate aminotransferase isoenzymes.

作者信息

Gracia R, Busquets M, Gil M, Cortés A, Bozal J

机构信息

Departament de Bioquímica i Fisiologia, Facultat de Química, Universitat de Barcelona, Spain.

出版信息

J Biochem Biophys Methods. 1987 Nov;15(2):63-9. doi: 10.1016/0165-022x(87)90034-0.

DOI:10.1016/0165-022x(87)90034-0

PMID:3437099

Abstract

A spectrophotometric assay is proposed to determine the levels of aspartate aminotransferase (AAT) isoenzymes from chicken liver by a steady-state kinetic method which depends on the differential inhibition of these isoenzyme forms by high concentrations of substrate 2-oxoglutarate at pH 6.2. The use of a standard curve permits the determination of the percentage of chicken liver c-AAT and m-AAT isoenzymes. This method yields results in good correlation with those achieved by different extent adipate inhibition and by differential centrifugation.

摘要

提出了一种分光光度法，通过稳态动力学方法测定鸡肝中天冬氨酸转氨酶（AAT）同工酶的水平，该方法依赖于在pH 6.2条件下高浓度底物2-氧代戊二酸对这些同工酶形式的差异抑制作用。使用标准曲线可以测定鸡肝c-AAT和m-AAT同工酶的百分比。该方法所得结果与通过不同程度的己二酸抑制和差速离心法所获得的结果具有良好的相关性。