Yonekura K, Kawakita T, Mitsuyama M, Miura O, Yumioka E, Suzuki A, Nomoto K
Traditional Chinese Medicine Research Laboratories, Kanebo Co. Ltd., Osaka, Japan.
Immunopharmacol Immunotoxicol. 1990;12(4):647-67. doi: 10.3109/08923979009019682.
Colony stimulating factor (CSF)-rich serum was obtained from mice injected intraperitoneally (ip) with shosaiko-to, a traditional Chinese herbal medicine. Transfer of the CSF-rich serum into naive mice augmented the resistance against Listeria monocytogenes. A dose-dependent induction of CSF was observed in mice given shosaiko-to via intravenous route as well as via intraperitoneal route. Since the serum CSF induction was observed in both lipopolysaccharide (LPS)-responder C3H/He mice and LPS-non-responder C3H/HeJ mice, the effect of shosaiko-to seemed to be independent of possibly contaminating LPS. The level of serum CSF induced by shosaiko-to in athymic nude mice was similar to that in control euthymic mice, and the induction of CSF was completely blocked by the previous administration of carrageenan, a selective macrophage-blocker. In mice treated ip with shosaiko-to CSF activity was detected in the peritoneal cavity, the site of injection, and the time course was similar to that of serum CSF induction. In a bone marrow culture system, the composition of colonies formed by shosaiko-to-induced CSF was similar to that formed by standard GM-CSF. The CSF activity was scarcely affected by treatment of the sera with anti-M-CSF antibody. These results suggest that shosaiko-to augments the host defense by inducing mainly GM-CSF, and that CSF is produced by cells of macrophage lineage. In addition, it was shown that CSF could be induced even after oral administration of herbal medicines.
富含集落刺激因子（CSF）的血清是从经腹腔注射（ip）小柴胡汤（一种传统中药）的小鼠中获得的。将富含CSF的血清转移到未接触过该血清的小鼠体内，可增强其对单核细胞增生李斯特菌的抵抗力。通过静脉途径以及腹腔途径给小鼠注射小柴胡汤后，均可观察到CSF呈剂量依赖性诱导。由于在脂多糖（LPS）反应性C3H/He小鼠和LPS非反应性C3H/HeJ小鼠中均观察到血清CSF诱导现象，小柴胡汤的作用似乎独立于可能污染的LPS。小柴胡汤在无胸腺裸鼠中诱导的血清CSF水平与对照有胸腺小鼠相似，并且CSF的诱导被预先给予的角叉菜胶（一种选择性巨噬细胞阻断剂）完全阻断。在经腹腔注射小柴胡汤治疗的小鼠中，在注射部位腹腔中检测到CSF活性，其时间进程与血清CSF诱导相似。在骨髓培养系统中，小柴胡汤诱导的CSF形成的集落组成与标准粒细胞-巨噬细胞集落刺激因子（GM-CSF）形成的集落组成相似。用抗巨噬细胞集落刺激因子（M-CSF）抗体处理血清后，CSF活性几乎不受影响。这些结果表明，小柴胡汤主要通过诱导GM-CSF增强宿主防御，并且CSF由巨噬细胞系细胞产生。此外，还表明即使口服草药也可诱导CSF。
