Hosoguchi Tomoya, Uchiyama Yuna, Komazawa Hinata, Yahata Masaki, Shimokawa Takashi, Tominaga Akiyoshi
Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, Shizuoka 422-8529, Japan.
Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
Plants (Basel). 2021 Jul 19;10(7):1480. doi: 10.3390/plants10071480.
Gerbera in vitro shoots were irradiated using three types of ion beams with different line energy transfers (LETs) to investigate the effective LET and absorbed doses for mutagenesis. Furthermore, genomic mutation analyses were conducted on the obtained mutants. Survival rate analysis showed a lower lethal dose 50% (LD) with ion beams with higher LETs. Trait/morphological mutations exhibited changes in the color and shape of petals and male sterility. Irradiation conditions with the highest growth change and trait/morphological mutation rates in each ion were C irradiation at 10 Gy, Ar irradiation at 5 Gy, and Fe irradiation at 5 Gy, with a range of absorbed dose of around LD to about 10 Gy lower. The highest trait/morphological mutation rate was 14.1% with Ar irradiation at 5 Gy, which was one of the criteria for ion beam irradiation of gerbera in vitro shoots. Furthermore, the genomic mutation in the flower color, petal shape, and male sterile mutants were confirmed by genotype analysis using Genotyping by Random Amplicon Sequencing-Direct technology. This is the first study to report the efficient production of gerbera mutants that could be analyzed. Our findings may lead to more efficient gerbera mutant production and analysis technology.
使用三种具有不同线能量传递（LET）的离子束对非洲菊离体芽进行辐照，以研究诱变的有效LET和吸收剂量。此外，对获得的突变体进行了基因组突变分析。存活率分析表明，LET较高的离子束的致死剂量50%（LD）较低。性状/形态突变表现为花瓣颜色和形状的变化以及雄性不育。每种离子中生长变化和性状/形态突变率最高的辐照条件分别为：10 Gy的碳离子（C）辐照、5 Gy的氩离子（Ar）辐照和5 Gy的铁离子（Fe）辐照，吸收剂量范围在LD左右至低约10 Gy。5 Gy的氩离子辐照下最高性状/形态突变率为14.1%，这是非洲菊离体芽离子束辐照的标准之一。此外，通过使用随机扩增子测序直接技术进行基因分型分析，证实了花色、花瓣形状和雄性不育突变体中的基因组突变。这是首次报道能够进行分析的非洲菊突变体高效生产的研究。我们的研究结果可能会带来更高效的非洲菊突变体生产和分析技术。
