Henan Key Laboratory of Ion-beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China.
Henan Key Laboratory of Ion-beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, China.
Ecotoxicol Environ Saf. 2018 Oct 30;162:341-347. doi: 10.1016/j.ecoenv.2018.07.013. Epub 2018 Jul 11.
Carbon ion beams irradiation as an important type of ionizing radiation is one of the major approaches used to create mutants in plants. This study investigated the role of carbon ion beams irradiation in mitigating cold stress in Arabidopsis thaliana seedlings. The results showed that 50-Gy carbon ion beam irradiation appeared stimulatory effects on root length and fresh weight in Arabidopsis seedlings under cold stress. In comparison with control, the content of hydrogen peroxide, the production rate of superoxide anion radical, hydroxyl radical generation activity, and malondialdehyde content were obviously decreased in 50-Gy carbon ion beam irradiated seedlings in response to cold stress. Moreover, irradiated 50-Gy carbon ion beam in Arabidopsis seedlings were significantly triggered the efficiency of antioxidant under cold stress. Furthermore, we investigated the expression of cold-related genes in irradiated and non-irradiated samples. Carbon ion beams irradiation up-regulated the expression levels of C-REPEAT BINDING FACTORS (CBFs), INDUCER OF CBF EXPRESSION 1 (ICE1), ICE2, CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 3 (CAMTA3) and cold-regulated COR genes, in response to cold stress. This study suggests that low-dose carbon ion beams irradiation can modulate the physiological responses and up-regulate cold signaling genes in mitigating cold stress in the growth of Arabidopsis seedlings.
碳离子束辐照作为一种重要的电离辐射类型，是植物诱变的主要方法之一。本研究探讨了碳离子束辐照在缓解拟南芥幼苗冷胁迫中的作用。结果表明，50Gy 碳离子束辐照对冷胁迫下拟南芥幼苗的根长和鲜重有刺激作用。与对照相比，50Gy 碳离子束辐照的拟南芥幼苗在冷胁迫下，过氧化氢含量、超氧阴离子自由基生成速率、羟自由基生成活性和丙二醛含量明显降低。此外，辐照的 50Gy 碳离子束在冷胁迫下显著触发了抗氧化剂的效率。此外，我们还研究了辐照和非辐照样品中冷相关基因的表达。碳离子束辐照上调了 C-重复结合因子（CBFs）、CBF 诱导因子 1（ICE1）、ICE2、钙调素结合转录激活因子 3（CAMTA3）和冷调节 COR 基因的表达水平，以应对冷胁迫。本研究表明，低剂量碳离子束辐照可以调节生理反应，上调冷信号基因，从而缓解拟南芥幼苗生长过程中的冷胁迫。
