Oi Y, Okuda T, Miyoshi H, Koishi H
Department of Food and Nutrition, Faculty of the Science of Living, Osaka City University, Japan.
J Nutr Sci Vitaminol (Tokyo). 1987 Aug;33(4):255-62. doi: 10.3177/jnsv.33.255.
The effect of restricted diets on protein metabolism was studied in obese rats (obesity had been induced by ad libitum feeding of a diet containing 30% fat and 25% casein). The obese rats were fed on one of three restricted diets, each containing 5% fat, for 2 weeks (restricted feeding groups); a high-protein diet (HPD, 50% casein), a standard-protein diet (SPD, 25% casein), or a low-protein diet (LPD, 5% casein). The food intake was restricted to 5 g per day per rat. On the eleventh day, the rats were given [15N]glycine orally, and 4 days later, they were killed. The restricted feeding groups all showed similar weight losses (about 100 g), 2 weeks after the start of the restricted diet. The 15N distribution in whole body was measured and results were compared with those of control rats given 5%- or 30%-fat diet ad libitum. The whole-body distribution of 15N in the HPD group was similar to that in the rats fed ad libitum although the diet intake was restricted. The results suggested that the amount of protein in a restricted diet is important for maintenance of protein metabolism in obese rats.
在肥胖大鼠中研究了限制饮食对蛋白质代谢的影响（肥胖是通过随意喂食含30%脂肪和25%酪蛋白的饮食诱导产生的）。将肥胖大鼠分为三组，每组喂食一种含5%脂肪的限制饮食，持续2周（限制喂养组）；分别为高蛋白饮食（HPD，50%酪蛋白）、标准蛋白饮食（SPD，25%酪蛋白）或低蛋白饮食（LPD，5%酪蛋白）。每只大鼠每天的食物摄入量限制为5克。在第11天，给大鼠口服[15N]甘氨酸，4天后将其处死。在开始限制饮食2周后，限制喂养组的大鼠体重均出现相似程度的减轻（约100克）。测量了全身的15N分布，并将结果与随意喂食5%或30%脂肪饮食的对照大鼠的结果进行比较。尽管饮食摄入量受到限制，但HPD组全身15N的分布与随意喂食的大鼠相似。结果表明，限制饮食中的蛋白质量对于维持肥胖大鼠的蛋白质代谢很重要。
