Central Laboratory, Chongqing University Three Gorges Hospital, Wanzhou, Chongqing, China.
Internal Medicine of Traditional Chinese Medicine, Chongqing University Three Gorges Hospital, Wanzhou, Chongqing, China.
Andrologia. 2021 Nov;53(10):e14195. doi: 10.1111/and.14195. Epub 2021 Aug 9.
Hexachlorobenzene is a widespread endocrine disruptor. However, the effect of hexachlorobenzene on the reproductive toxicity of male animals is not described in detail. To investigate the toxic effects of hexachlorobenzene in mouse testes, hexachlorobenzene (100, 400 and 1,600 mg/kg) is fed to mice. The morphology of the testes was analysed by haematoxylin and eosin staining. We also investigated the expression of biomarkers for oxidative stress. Database screening identified proteins that interact with hexachlorobenzene and the aryl hydrocarbon receptor, a weak ligand of hexachlorobenzene. Gene enrichment analysis and protein-protein interaction analyses were also performed. Real-time PCR detected the expression levels of the aryl hydrocarbon receptor in four different stages of testicular cells. We identified significantly increased activity levels of superoxide dismutase (p < 0.05) and catalase (p < 0.05) in mouse testes that had been subjected to oxidative damage. The cell thickness and the number of cell layers in the seminiferous tubules had decreased by varying degrees after the hexachlorobenzene treatment. Particularly, cytokines and proteins involved in transcriptional regulation showed enrichment. The highest levels of aryl hydrocarbon receptor expression were detected in the spermatocytic cell line. Hexachlorobenzene exposure caused testicular damage in mice. The toxicity characteristics of hexachlorobenzene were not dose-dependent.
六氯苯是一种广泛存在的内分泌干扰物。然而,六氯苯对雄性动物生殖毒性的影响尚未详细描述。为了研究六氯苯对小鼠睾丸的毒性作用,我们用六氯苯(100、400 和 1600mg/kg)对小鼠进行灌胃。通过苏木精-伊红染色分析睾丸的形态。我们还研究了氧化应激生物标志物的表达。数据库筛选鉴定出与六氯苯和芳香烃受体相互作用的蛋白质,后者是六氯苯的弱配体。进行了基因富集分析和蛋白质-蛋白质相互作用分析。实时 PCR 检测了四组不同睾丸细胞中芳香烃受体的表达水平。我们发现,暴露于六氯苯的小鼠睾丸中超氧化物歧化酶(p<0.05)和过氧化氢酶(p<0.05)的活性水平显著升高。经六氯苯处理后,生精小管的细胞厚度和细胞层数均有不同程度的减少。特别是转录调控相关的细胞因子和蛋白表现出富集。芳香烃受体表达水平最高的是精母细胞系。六氯苯暴露导致小鼠睾丸损伤。六氯苯的毒性特征与剂量无关。