National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, China.
Key Laboratory of Soil Contamination Bioremediation of Zhejiang Province, Zhejiang A&F University, Hangzhou, China.
J Sci Food Agric. 2022 Mar 15;102(4):1405-1414. doi: 10.1002/jsfa.11473. Epub 2021 Sep 10.
Tea plants have high nitrogen (N) consumptions, whereas molecular and physiological responses of tea plants to N recovery are still unclear.
By using non-invasive micro-test technology (NMT), N tracer technique, ultra-performance liquid chromatography (UPLC), and transcriptome sequencing technology, we investigated the N recovery-induced changes in N absorptions, N tissue distributions, contents of free amino acids (FAAs), and global transcription of the low-N tolerant and intolerant tea genotypes [i.e. Wuniuzao (W) and Longjing43 (L)]. The results showed that the phenotype of Wuniuzao was better than that of Longjing43 under low-N condition. The N absorption and utilization of Wuniuzao were superior to Longjing43 under N recovery. The γ-aminobutyric acid (GABA) ratio (N recovery/N deficiency) in the root of Wuniuzao was significantly higher than that of Longjing43, while the glutamic acid ratio in the root of Wuniuzao was significantly lower than that of Longjing43. This findings suggested that Wuniuzao tended to enhance the GABA synthesis, while Longjing43 tended to inhibit the GABA synthesis under N recovery. The key genes in response to N recovery in Wuniuzao included N transport (AMT and NRT), N transformation (NR, NirA, and GAD), and amino acid transport (GAT) genes. In addition, some ribosome and flavonoid biosynthesis genes might help to maintain proteome homeostasis.
The N absorption and transport, and the conversion abilities of key amino acids (Glu and GABA) might improve the adaptability of tea plants to N recovery, which provided a basis for the breeding of N efficient tea varieties. © 2021 Society of Chemical Industry.
茶树具有较高的氮（N）消耗，而茶树对 N 回收的分子和生理响应仍不清楚。
利用非侵入性微量测试技术（NMT）、N 示踪技术、超高效液相色谱（UPLC）和转录组测序技术，我们研究了 N 回收引起的 N 吸收、N 组织分布、游离氨基酸（FAAs）含量和低 N 耐受和不耐受茶树基因型（即乌牛早（W）和龙井 43（L））的整体转录变化。结果表明，低 N 条件下乌牛早的表型优于龙井 43。在 N 回收下，乌牛早的 N 吸收和利用优于龙井 43。乌牛早根中的γ-氨基丁酸（GABA）比（N 回收/N 缺乏）显著高于龙井 43，而乌牛早根中的谷氨酸比显著低于龙井 43。这表明乌牛早倾向于增强 GABA 合成，而龙井 43则倾向于抑制 GABA 合成。乌牛早对 N 回收的响应关键基因包括 N 转运（AMT 和 NRT）、N 转化（NR、NirA 和 GAD）和氨基酸转运（GAT）基因。此外，一些核糖体和类黄酮生物合成基因可能有助于维持蛋白质组平衡。
N 的吸收和转运以及关键氨基酸（Glu 和 GABA）的转化能力可能提高了茶树对 N 回收的适应性，为培育高效 N 利用的茶树品种提供了依据。© 2021 化学工业协会。
