First Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China.
Department of Andrology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
Andrologia. 2021 Nov;53(10):e14198. doi: 10.1111/and.14198. Epub 2021 Aug 10.
This study aimed to verify that Xuefu Zhuyu decoction (XFZYD) can improve asthenozoospermia caused by asthma, and explore its potential mechanism. Ovalbumin solution is used to induce asthma rat models. Sperm concentration and motility are used to evaluate semen quality. Immunohistochemistry (IHC), Western blotting and real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) are used to detect proteins and mRNA related to rat testis tissue. Haematoxylin and eosin (H&E) staining was used to observe changes in testicular tissues. Through network pharmacology, eriodictyol, 18-β-glycyrrhetinic acid, naringenin, chrysin and Hispidulin were prominent active ingredients of XFZYD. We found that XFZYD regulates the expression levels of albumin (ALB), vascular endothelial growth factor A (VEGFA), interleukin 6 (IL-6) protein and mRNA, thereby improving the histopathological morphology of the testis, increasing the concentration and motility of spermatozoa. We suggest that future research can increase the detection of hormones and oxidative stress and other related indicators, so as to conduct more in-depth exploration.
本研究旨在验证血府逐瘀汤（XFZYD）可改善哮喘引起的弱精症，并探讨其潜在机制。采用卵白蛋白溶液诱导哮喘大鼠模型。精子浓度和活力用于评估精液质量。免疫组织化学（IHC）、Western blot 和实时逆转录定量聚合酶链反应（RT-qPCR）用于检测与大鼠睾丸组织相关的蛋白和 mRNA。苏木精和伊红（H&E）染色用于观察睾丸组织的变化。通过网络药理学，研究发现，柚皮苷、18-β-甘草次酸、柚皮素、白杨素和高车前素是 XFZYD 的主要活性成分。我们发现 XFZYD 可调节白蛋白（ALB）、血管内皮生长因子 A（VEGFA）、白细胞介素 6（IL-6）蛋白和 mRNA 的表达水平，从而改善睾丸的组织病理学形态，增加精子浓度和活力。我们建议未来的研究可以增加对激素和氧化应激等相关指标的检测，以进行更深入的探索。
