First Clinical Medical College, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.
Andrology Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, People's Republic of China.
Drug Des Devel Ther. 2021 Mar 19;15:1245-1259. doi: 10.2147/DDDT.S296901. eCollection 2021.
Jiawei Shengjiang Powder (JWSJP) is a classical Chinese medicinal formula, which has been widely applied in the treatment of asthma and complications for many years due to its curative effect.
To verify the effect of JWSJP in improving abnormal sperm motility caused by asthma and to explore its potential mechanism.
The active compounds of JWSJP were obtained from high performance liquid chromatography tandem mass spectrometry and the Traditional Chinese Medicine System Pharmacology. The key active components and targets of JWSJP were predicted based on network pharmacological analysis and bioinformatics research. Rats were randomly divided into normal, model and treatment groups. The rat model of allergic asthma was induced by intraperitoneal injection of ovalbumin solution. The experiment judged improvement of semen quality by evaluating sperm motility, and detected the expression of related proteins in testicular tissue of Sprague-Dawley rats by RT-qPCR and Western blot methods. Hematoxylin and eosin (HE) staining was used to observe the changes in testicular tissue structure in rats.
Through the analysis of network pharmacology and bioinformatics, it was found that beta-sitosterol, quercetin, gallic acid, pelargonidin and kaempferol were the key active components of Jiawei Shengjiang Powder. Tumor necrosis factor (TNF), interleukin-6 (IL-6) and insulin (INS) genes are crucial targets of JWSJP in the treatment of spermatogenic dysfunction caused by acute asthma. After 8 weeks of intervention, compared with the model group, the treatment group had significantly improved sperm motility (P < 0.05). There were significant differences in TNF, IL6, and INS proteins in the treatment group, and the HE staining of testicular tissue structure in the treatment group was significantly improved.
JWSJP can improve the abnormal sperm motility induced by asthma, and its mechanism may be related to the expression of related proteins and mRNA of TNF, IL6, and INS.
加味生精粉（JWSJP）是一种经典的中药方剂，由于其疗效，多年来已广泛应用于哮喘及并发症的治疗。
验证 JWSJP 改善哮喘引起的异常精子活力的效果，并探讨其潜在机制。
采用高效液相色谱串联质谱法和中药系统药理学法获得 JWSJP 的活性化合物。基于网络药理学分析和生物信息学研究预测 JWSJP 的关键活性成分和靶点。将大鼠随机分为正常组、模型组和治疗组。采用腹腔注射卵清蛋白溶液诱导大鼠过敏性哮喘模型。实验通过评估精子活力来判断精液质量的改善，并通过 RT-qPCR 和 Western blot 方法检测 Sprague-Dawley 大鼠睾丸组织中相关蛋白的表达。采用苏木精-伊红（HE）染色观察大鼠睾丸组织结构的变化。
通过网络药理学和生物信息学分析，发现 beta-谷甾醇、槲皮素、没食子酸、矢车菊素和山奈酚是加味生精粉的关键活性成分。肿瘤坏死因子（TNF）、白细胞介素-6（IL-6）和胰岛素（INS）基因是 JWSJP 治疗急性哮喘引起的生精功能障碍的关键靶点。干预 8 周后，与模型组相比，治疗组的精子活力明显提高（P < 0.05）。治疗组 TNF、IL6 和 INS 蛋白存在显著差异，且治疗组睾丸组织结构 HE 染色明显改善。
JWSJP 可改善哮喘引起的异常精子活力，其机制可能与 TNF、IL6 和 INS 相关蛋白和 mRNA 的表达有关。
