基于熵驱动的催化反应驱动 DNA 马达，用于同时检测食品中的赭曲霉毒素 A 和氯霉素。
An entropy driven catalytic reaction powered DNA motor for simultaneous detection of ochratoxin A and chloramphenicol in food.
机构信息
School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.
Chongqing Key Laboratory of Catalysis and New Environmental Materials, College of Environment and Resources, Chongqing Technology and Business University, Chongqing 400067, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jan 5;264:120264. doi: 10.1016/j.saa.2021.120264. Epub 2021 Aug 6.
An entropy driven catalytic reaction powered DNA motor was proposed for simultaneous detection of ochratoxin A (OTA) and chloramphenicol (CAP) in food. The dumbbell hairpin structure was formed by the two aptamers of OTA and CAP. The dumbbell hairpin can be opened by the interaction of OTA and CAP with their aptamers. The tails of the end of dumbbell hairpin sequence can induce the entropy driven catalytic reactions on the AuNPs, causing the sustained releasing of the fluorophore labeled DNA sequences. The recovery of fluorescent intensities can be used for quantitative detection of OTA and CAP. The limit of detection reached 2 pM for OTA and 6 pM for CAP respectively, which was great improved by entropy driven amplification of the self-powdered DNA motor. This strategy is simple and sensitive and only needs one-step operation. It exhibits promising potentiality in food quality control and food security supervision.
提出了一种基于熵驱动催化反应的 DNA 分子马达，用于同时检测食品中的赭曲霉毒素 A（OTA）和氯霉素（CAP）。该分子马达由 OTA 和 CAP 的两个适体形成哑铃状发夹结构。哑铃状发夹可以通过 OTA 和 CAP 与其适体的相互作用而打开。哑铃状发夹末端的序列尾巴可以在 AuNPs 上诱导熵驱动的催化反应，导致荧光标记的 DNA 序列的持续释放。荧光强度的恢复可用于定量检测 OTA 和 CAP。该方法对 OTA 和 CAP 的检测限分别达到 2 pM 和 6 pM，通过自组装 DNA 分子马达的熵驱动放大得到了极大的改善。该策略简单灵敏，仅需一步操作，在食品质量控制和食品安全监管方面具有广阔的应用前景。
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