Department of Life Sciences and Chemistry, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
Institute of Chemistry of New Materials, Universität Osnabrück, Barbarastr. 7, 49080 Osnabrück, Germany.
Org Biomol Chem. 2021 Oct 14;19(39):8521-8529. doi: 10.1039/d1ob01304a.
The accurate determination of ultra-high binding affinities in supramolecular host-guest chemistry is a challenging endeavour because direct binding titrations are generally limited to affinities <10 M due to sensitivity constraints of common titration methods. To determine higher affinities, competitive titrations are usually performed, in which one compound with a well established binding affinity serves as a reference. Herein, we propose a reference scale for such competitive titrations with the host cucurbit[7]uril (CB7) comprising binding affinities in the range from 10 to 10 M. The suggested reference compounds are commercially available and will aid in the future determination of CB7 binding affinities for stimuli-responsive host-guest systems.
超分子主客体化学中超高结合亲和力的精确测定是一项具有挑战性的工作，因为由于常见滴定方法的灵敏度限制，直接结合滴定通常仅限于亲和力 <10 M。为了确定更高的亲和力，通常进行竞争性滴定，其中一种具有良好确立结合亲和力的化合物用作参考。在此，我们提出了一种使用葫芦[7]脲（CB7）作为主体的此类竞争性滴定的参考标度，其结合亲和力范围为 10 至 10 M。建议的参考化合物可商购，将有助于未来对响应性主客体系统的 CB7 结合亲和力的测定。
