The University of Haripur, Department of Microbiology, Haripur, Pakistan.
University of Veterinary and Animal Sciences, University Diagnostic Lab, Lahore, Pakistan.
Braz J Biol. 2021 Aug 9;83:e246514. doi: 10.1590/1519-6984.246514. eCollection 2021.
The poultry sector in Pakistan is contributing mainly in bridging gap between demand and supply for protein. Mycoplasma gallisepticum is an emerging bacterium causing serious problems in poultry industry of Pakistan. A cross-sectional study was conducted to evaluate the M. gallisepticum load in poultry populated regions of Pakistan. Total 600 serum and 600 swab samples were collected, 200 from each broiler, layers and breeders poultry in Rawalpindi and Abbottabad districts. Serum samples were analyzed through ELISA for seroprevalence. Swabs were cultured on Frey's medium followed by PCR and partial mgc2 gene sequencing. Results of seroprevalence of M. gallisepticum showed that layers (75%, n=150) are more positive as compared to breeders (70%, n=140) and broilers (50%, n=100). Typical colonies of the M. gallisepticum were observed in breeder (26.5%), followed by layer (21%) and broilers (9%). A total of 37.1% (n=42) samples were identified positive through PCR out of total 113 cultured based positive samples. A total of six M. gallisepticum isolates of current study showed 98-99 percent similarity with previously reported isolates on the basis of mgc2 gene partial sequencing. The M. gallisepticum was found highly prevalent in different poultry breads. Results of this study would add into basic data and provide a direction for livestock sector to strengthen a control strategy for mycoplasmosis in poultry farms.
巴基斯坦的家禽业主要负责弥补蛋白质供需之间的缺口。滑液囊支原体是一种新兴的细菌，给巴基斯坦的家禽业带来了严重的问题。本研究采用横断面研究方法，评估了巴基斯坦家禽养殖区的滑液囊支原体负荷。共采集了 600 份血清和 600 份拭子样本，其中肉鸡、蛋鸡和种鸡各 200 份，分别来自拉瓦尔品第和阿伯塔巴德地区。通过 ELISA 检测血清样本的抗体流行率，对拭子进行弗雷氏培养基培养，然后进行 PCR 和部分 mgc2 基因测序。血清抗体流行率结果显示，蛋鸡（75%，n=150）比种鸡（70%，n=140）和肉鸡（50%，n=100）更易感染。在种鸡（26.5%）、蛋鸡（21%）和肉鸡（9%）中观察到典型的滑液囊支原体菌落。在总共 113 个培养阳性样本中，有 37.1%（n=42）通过 PCR 鉴定为阳性。本研究的 6 株滑液囊支原体分离株在 mgc2 基因部分测序的基础上，与之前报道的分离株具有 98-99%的相似性。不同家禽品种中均高度流行滑液囊支原体。本研究结果将为基础数据提供补充，并为畜牧业部门加强禽场支原体病防控策略提供方向。
