Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
Open Vet J. 2022 May-Jun;12(3):329-334. doi: 10.5455/OVJ.2022.v12.i3.4. Epub 2022 May 21.
is a significant microorganism of poultry, which can cause respiratory infections and synovial inflammation, bringing about huge financial misfortunes to poultry workmanship worldwide.
The goal of existing research was to determine the infection rate of (MG) from chronic respiratory disease cases among broilers fields in Mosul/ Iraq using the polymerase chain reaction (PCR) technique.
All 92 lungs samples were collected from broilers with classical respiratory signs in different regions of the Nineveh governorate for 3 months from February to April 2021.
PCR tests were performed using two couple primers, one for the qualitative amplification of 16S rRNA genes (285 base pairs) in spp. and the second couple for the detection of (580 base pairs). Among the samples obtained from broilers, 87 (94.7%) were positive for and 79 (85.9%) were positive for .
Our results showed that MG infection in broiler chickens leads to serious clinical symptoms and severe lesions. The rate of isolation in this study is high despite the short lifespan of broiler chickens.
是家禽的一种重要微生物，可引起呼吸道感染和滑膜炎，给全球家禽养殖业带来巨大的经济损失。
本研究旨在利用聚合酶链反应（PCR）技术确定伊拉克摩苏尔地区肉鸡场慢性呼吸道疾病病例中（MG）的感染率。
从 2021 年 2 月至 4 月的 3 个月内，从尼尼微省不同地区具有典型呼吸道症状的肉鸡中采集了 92 个肺样本。
使用两对引物对样本进行了 PCR 检测，一对引物用于定性扩增 spp. 的 16S rRNA 基因（285 个碱基对），另一对引物用于检测 （580 个碱基对）。从肉鸡中获得的样本中，87 份（94.7%）为阳性，79 份（85.9%）为阳性。
我们的结果表明，MG 感染肉鸡可导致严重的临床症状和严重的病变。尽管肉鸡的寿命很短，但本研究中分离出的 MG 率很高。
