Kemper C M, O'Connor D T, Westlund K N
Department of Anatomy & Neurosciences, University of Texas Medical Branch, Galveston 77550.
Neuroscience. 1987 Dec;23(3):981-9. doi: 10.1016/0306-4522(87)90173-4.
The distribution of catecholaminergic cells in the human pons and medulla was illustrated by immunocytochemistry using a polyclonal antibody directed against the catecholamine synthetic enzyme, dopamine-beta-hydroxylase. The antibody specifically recognizes dopamine-beta-hydroxylase in putative adrenergic and noradrenergic neurons. The adrenergic and noradrenergic neurons are found in the brain stem from caudal levels of the medulla through the caudalmost levels of the midbrain. Large numbers of dopamine-beta-hydroxylase-positive cells were observed in cell groups of the medulla. Additionally, dopamine-beta-hydroxylase-positive cells were concentrated in the nucleus locus coeruleus and the nucleus subcoeruleus in the pons. These studies confirm that immunocytochemical localization of dopamine-beta-hydroxylase can be used to identify noradrenergic and adrenergic neurons and their terminal varicosities in the pons and medulla in routine autopsy material. These studies have also illustrated that the distribution of dopamine-beta-hydroxylase in the adult human brain is comparable to the distribution in other species.
利用针对儿茶酚胺合成酶多巴胺-β-羟化酶的多克隆抗体，通过免疫细胞化学方法展示了人脑桥和延髓中儿茶酚胺能细胞的分布。该抗体能特异性识别假定的肾上腺素能和去甲肾上腺素能神经元中的多巴胺-β-羟化酶。从延髓的尾端水平到中脑最尾端水平的脑干中均可发现肾上腺素能和去甲肾上腺素能神经元。在延髓的细胞群中观察到大量多巴胺-β-羟化酶阳性细胞。此外，多巴胺-β-羟化酶阳性细胞集中在脑桥的蓝斑核和蓝斑下核。这些研究证实，多巴胺-β-羟化酶的免疫细胞化学定位可用于在常规尸检材料中识别脑桥和延髓中的去甲肾上腺素能和肾上腺素能神经元及其终末膨体。这些研究还表明，多巴胺-β-羟化酶在成人大脑中的分布与其他物种中的分布相当。
