State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, China.
School of Marine Science, Ningbo University, Ningbo, China.
Food Funct. 2021 Oct 4;12(19):9030-9042. doi: 10.1039/d1fo01884a.
Hyperuricaemia is a disease associated with elevated serum uric acid content, which has emerged rapidly in recent decades. The drugs used to treat clinical hyperuricaemia have side effects, and their safety is poor. However, anserine is a natural carnosine derivative that shows an anti-hyperuricaemic effect. A previous study demonstrated that anserine inhibits uric acid synthesis and promotes uric acid excretion, but there is no evidence regarding the effect of anserine from the perspective of the gut microbiota. In this study, the anti-hyperuricaemic and anti-inflammatory effects of anserine were explored in a diet-induced hyperuricaemic mouse model. Anserine alleviated hyperuricaemia and renal inflammation phenotypes, inhibited uric acid biosynthesis, promoted uric acid excretion, and inhibited NLRP3 inflammasome and TLR4/MyD88/NF-κB signalling pathway activation. The results showed that the anti-hyperuricaemic effect of anserine was dependent on the gut microbiota in the germ-free mice experiment. Furthermore, anserine treatment reversed gut microbiota dysbiosis, repaired the intestinal epithelial barrier and increased short-chain fatty acid production. Moreover, the anti-hyperuricaemic effect of anserine was transmissible by transplanting the faecal microbiota from anserine-treated mice, indicating that the protective effects were at least partially mediated by the gut microbiota. Thus, we identified a new and safe prebiotic material to alleviate hyperuricaemia and provided ideas for the development of oligopeptides.
高尿酸血症是一种与血清尿酸含量升高相关的疾病，在近几十年来迅速出现。用于治疗临床高尿酸血症的药物有副作用，且安全性较差。然而，鹅肌肽是一种天然肌肽衍生物，具有抗高尿酸血症作用。先前的研究表明，鹅肌肽抑制尿酸合成并促进尿酸排泄，但从肠道微生物群的角度来看，尚无关于鹅肌肽作用的证据。在这项研究中，在饮食诱导的高尿酸血症小鼠模型中探索了鹅肌肽的抗高尿酸血症和抗炎作用。鹅肌肽缓解了高尿酸血症和肾脏炎症表型，抑制了尿酸生物合成，促进了尿酸排泄，并抑制了 NLRP3 炎性体和 TLR4/MyD88/NF-κB 信号通路的激活。结果表明，在无菌小鼠实验中，鹅肌肽的抗高尿酸血症作用依赖于肠道微生物群。此外，鹅肌肽治疗逆转了肠道微生物群失调，修复了肠上皮屏障并增加了短链脂肪酸的产生。此外，鹅肌肽处理通过移植鹅肌肽治疗小鼠的粪便微生物群可以传递抗高尿酸血症作用，表明保护作用至少部分通过肠道微生物群介导。因此，我们确定了一种新的安全的益生元物质来缓解高尿酸血症，并为寡肽的开发提供了思路。
