State Key Laboratory of Animal Nutrition, Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, China Agricultural University, Beijing, China.
College of Life Science and Bioengineering, Beijing Jiaotong University, Beijing, China.
J Nutr. 2021 Nov 2;151(11):3391-3399. doi: 10.1093/jn/nxab250.
Probiotics are beneficial in intestinal disorders. However, the benefits of Lactobacillus johnsonii in experimental colitis remain unknown.
This study aimed to investigate the benefits of L. johnsonii against Citrobacter rodentium-induced colitis.
Thirty-six 5-wk-old female C57BL/6J mice were randomly assigned to 3 groups (n = 12): control (Ctrl) group, Citrobacter rodentium treatment (CR) group (2 × 109 CFU C. rodentium), and Lactobacillus johnsonii and Citrobacter rodentium cotreatment (LJ + CR) group (109 CFU L. johnsonii with C. rodentium). Colon length, mucosal thickness, proinflammatory cytokine genes, and endoplasmic reticulum stress were tested.
The CR group had greater spleen weight, mucosal thickness, and Ki67+ cells (0.4-4.7 times), and a 23.8% shorter colon length than the Ctrl group, which in the LJ + CR group were 22.4%-77.6% lower and 30% greater than in the CR group, respectively. Relative to the Ctrl group, serum proinflammatory cytokines and immune cell infiltration were greater by 0.3-1.6 times and 6.2-8.8 times in the CR group, respectively; relative to the CR group, these were 19.9%-61.9% and 69.5%-84.2% lower in the LJ + CR group, respectively. The mRNA levels of lysozyme (Lyz) and regenerating islet-derived protein III were 22.7%-36.5% lower and 1.5-2.7 times greater in the CR group than in the Ctrl group, respectively, whereas they were 22.2%-25.7% greater and 57.2%-76.9% lower in the LJ + CR group than in the CR group, respectively. Cell apoptosis was 11.9 times greater in the CR group than in the Ctrl group, and 87.4% lower in the LJ + CR group than in the CR group. Consistently, the protein abundances of C/EBP homologous protein (CHOP), cleaved caspase 1 and 3, activating transcription factor 6α (ATF6A), and phospho-inositol-requiring enzyme 1α (P-IRE1A) were 0.3-2.1 times greater in the CR group and 31.1%-60.4% lower in the LJ + CR group. All these indexes did not differ between the Ctrl and LJ + CR groups, except for CD8+ T lymphocytes and CD11b+ and F4/80+ macrophages (1-1.5 times greater in LJ + CR) and mRNA concentration of Lyz2 (20.1% lower in LJ + CR).
L. johnsonii supplementation is a promising nutritional strategy for preventing C. rodentium-induced colitis in mice.
益生菌对肠道疾病有益。然而，鼠李糖乳杆菌（Lactobacillus johnsonii）在实验性结肠炎中的益处尚不清楚。
本研究旨在探讨鼠李糖乳杆菌（L. johnsonii）对柠檬酸杆菌（Citrobacter rodentium）诱导的结肠炎的作用。
将 36 只 5 周龄雌性 C57BL/6J 小鼠随机分为 3 组（每组 12 只）：对照组（Ctrl）、柠檬酸杆菌（Citrobacter rodentium）处理组（CR）（2×109 CFU C. rodentium）和鼠李糖乳杆菌和柠檬酸杆菌共同处理组（LJ + CR）（109 CFU 鼠李糖乳杆菌与 C. rodentium）。检测结肠长度、黏膜厚度、促炎细胞因子基因和内质网应激。
与对照组相比，CR 组的脾重、黏膜厚度和 Ki67+细胞（0.4-4.7 倍）增加，结肠长度缩短 23.8%，而 LJ + CR 组分别降低 22.4%-77.6%和增加 30%。与对照组相比，CR 组血清促炎细胞因子和免疫细胞浸润分别增加 0.3-1.6 倍和 6.2-8.8 倍，而 LJ + CR 组分别降低 19.9%-61.9%和 69.5%-84.2%。与对照组相比，CR 组溶菌酶（Lyz）和再生胰岛衍生蛋白 III 的 mRNA 水平分别降低 22.7%-36.5%和增加 1.5-2.7 倍，而 LJ + CR 组分别增加 22.2%-25.7%和增加 57.2%-76.9%。与对照组相比，CR 组细胞凋亡增加 11.9 倍，LJ + CR 组减少 87.4%。同样，CR 组 C/EBP 同源蛋白（CHOP）、切割的半胱天冬酶 1 和 3、激活转录因子 6α（ATF6A）和磷酸肌醇需要酶 1α（P-IRE1A）的蛋白丰度增加 0.3-2.1 倍，而 LJ + CR 组降低 31.1%-60.4%。除 CD8+T 淋巴细胞、CD11b+和 F4/80+巨噬细胞（LJ + CR 组增加 1-1.5 倍）和溶菌酶 2（Lyz2）mRNA 浓度（LJ + CR 组降低 20.1%）外，所有这些指标在对照组和 LJ + CR 组之间均无差异。
鼠李糖乳杆菌（L. johnsonii）补充剂是预防柠檬酸杆菌（C. rodentium）诱导的结肠炎的一种有前途的营养策略。
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