Graduate Program in Cardiovascular Sciences, Fluminense Federal University (UFF), Niterói, RJ, Brazil.
Department of Marine Biology, Fluminense Federal University (UFF), Niterói, RJ, Brazil.
Mol Nutr Food Res. 2021 Oct;65(19):e2100374. doi: 10.1002/mnfr.202100374. Epub 2021 Aug 22.
Resistant starch type-2 (RS2) can mitigate inflammation and oxidative stress in hemodialysis (HD) patients. However, there is still a lack of knowledge on the impact of the RS2 on the gut microbiota community in these patients. Thus, this study aims to evaluate the effects of enriched RS2 cookies on the gut microbiome in HD patients.
This comprises a randomized, double-blind, placebo-controlled trial of age-, sex-, and BMI-matched patients and controls. The RS2 group receives enriched RS2 cookies (16 g d of Hi-Maize 260, Ingredion) for 4 weeks, while the placebo group received cookies made with manioc flour. Fecal microbiota composition is evaluated by the 16S ribosomal RNA gene. Analysis of the microbiota reveals that Pielou's evenness is significantly decreased after RS2 supplementation. Notably, it is observed that RS2 intervention upregulates significantly 8 Amplicon Sequencing Variants (ASV's), including Roseburia and Ruminococcus gauvreauii, which are short-chain fatty acids (SCFA) producers. Furthermore, it is associated with the downregulation of 11 ASVs, such as the pro-inflammatory Dialister.
RS2 intervention for 4 weeks in HD patients effectively alters SCFA producers in the gut microbiota, suggesting that it could be a good nutritional strategy for patients with chronic kidney disease (CKD) on HD.
抗性淀粉 2 型（RS2）可减轻血液透析（HD）患者的炎症和氧化应激。然而，关于 RS2 对这些患者肠道微生物群落的影响，我们的了解仍然有限。因此，本研究旨在评估富含 RS2 的曲奇饼干对 HD 患者肠道微生物组的影响。
这是一项随机、双盲、安慰剂对照试验，纳入了年龄、性别和 BMI 匹配的患者和对照组。RS2 组每天接受 16 克高直链玉米淀粉 260（英格莱）强化的 RS2 曲奇饼干，而安慰剂组则接受用木薯粉制成的曲奇饼干。通过 16S 核糖体 RNA 基因评估粪便微生物群组成。微生物群分析表明，补充 RS2 后皮氏均匀度显著降低。值得注意的是，RS2 干预显著上调了 8 个扩增子序列变异（ASV），包括 Roseburia 和 Ruminococcus gauvreauii，它们是短链脂肪酸（SCFA）的产生菌。此外，还与 11 个 ASV 的下调相关，如促炎的 Dialister。
在 HD 患者中进行 4 周的 RS2 干预可有效改变肠道微生物群中的 SCFA 产生菌，这表明它可能是 HD 慢性肾脏病（CKD）患者的一种良好营养策略。
