Biochemistry, Department of Chemical Sciences, Godfrey Okoye University, P.M.B 01014, Thinkers Corner, Enugu Nigeria.
Department of Biological and Environmental Sciences, Faculty of Sciences, Walter Sisulu University, Mthatha 5117, South Africa.
Afr Health Sci. 2021 Mar;21(1):150-158. doi: 10.4314/ahs.v21i1.20.
Though sickle cell anaemia (SCA) is known to promote oxidative stress, there is paucity of information on the relationship between oxidative stress and vaso-occlusive crisis (VOC).
This study was undertaken to evaluate the relationship of oxidative stress and antioxidant response with VOC in SCA.
A cross-sectional case-control study was carried out at University of Nigeria Teaching Hospital (UNTH), Ituku-Ozalla, Enugu Nigeria involving 116 individuals which included 36 SCA subject, 40 sickle cell carriers (AS) and 40 healthy individuals (AA). Baseline information as well as the frequency of VOC was obtained from the participants and anaemia as well as oxidative stress and antioxidant indices were assessed in blood.
Anaemia was prevalent (88.9 %) in SCA individuals compared to AS (52.5%) and AA (47.5 %) individuals. Nitric oxide scavenging (NOS) and superoxide dismutase (SOD) activities as well as glutathione level were significantly (p<0.005) lower while catalase activity was higher in SCA individuals compared to controls (AA and AS). Higher malondialdehyde (MDA) level was associated with very severe VOC while low level of NOS activity was associated with severe VOC in SCA individuals.
Sickle cell anaemia exhibited oxidative stress and alteration in the levels of antioxidant indices which was possibly associated with vaso-occlusive crisis.
尽管镰状细胞贫血症（SCA）已知会促进氧化应激，但关于氧化应激与血管阻塞性危象（VOC）之间的关系的信息却很少。
本研究旨在评估氧化应激和抗氧化反应与 SCA 中 VOC 的关系。
在尼日利亚恩古州伊图库-奥扎拉的尼日利亚大学教学医院（UNTH）进行了一项横断面病例对照研究，共纳入 116 名个体，包括 36 名 SCA 患者、40 名镰状细胞携带者（AS）和 40 名健康个体（AA）。从参与者那里获得了基线信息以及 VOC 的频率，并在血液中评估了贫血以及氧化应激和抗氧化指标。
与 AS（52.5%）和 AA（47.5%）个体相比，SCA 个体中贫血更为普遍（88.9%）。与对照组（AA 和 AS）相比，SCA 个体中的一氧化氮清除酶（NOS）和超氧化物歧化酶（SOD）活性以及谷胱甘肽水平显著降低（p<0.005），而过氧化氢酶活性升高。在 SCA 个体中，较高的丙二醛（MDA）水平与非常严重的 VOC 相关，而 NOS 活性较低与严重的 VOC 相关。
镰状细胞贫血症表现出氧化应激和抗氧化指标水平的改变，这可能与血管阻塞性危象有关。
