两种人静脉注射免疫球蛋白制剂对感染包膜化金黄色葡萄球菌菌株的实验性保护作用。

Protective activity of two human intravenous immunoglobulin preparations in experimental infection with an encapsulated Staphylococcus aureus strain.

作者信息

Vassilev T, Petrova R, Bozadjiev L, Peeva Z, Bineva I

机构信息

Institute of Infectious and Parasitic Diseases, Medical Academy, Sofia, Bulgaria.

出版信息

Acta Microbiol Hung. 1987;34(2):139-45.

PMID:3439436

Abstract

The protective effect of two different human immunoglobulin preparations for intravenous use and one specific staphylococcal immunoglobulin for intramuscular application were compared in mice infected with the capsular Staphylococcus aureus Smith strain. Immunovenin is produced by partial fragmentation of IgG with plasmin; it contains about 60% intact IgG and 40% Fab and Fc fragments. Immunovenin-intact is produced by a polyethylene glycol (mol wt 6000) fractionation method followed by ion exchange chromatography and contains more than 95% intact IgG molecules. A specific staphylococcal immunoglobulin is obtained by a rivanol/ethanol fractionation method from selected sera with high anti-alpha toxin level. All three types of immunoglobulins induced similar degree of protection when their effect was determined as activity (ED50) per gram immunoglobulin.

摘要

在感染了包膜金黄色葡萄球菌史密斯菌株的小鼠中，比较了两种不同的静脉用人免疫球蛋白制剂和一种特定的肌肉注射用葡萄球菌免疫球蛋白的保护作用。免疫静脉球蛋白是通过用纤溶酶对IgG进行部分裂解产生的；它含有约60%的完整IgG以及40%的Fab和Fc片段。完整免疫静脉球蛋白是通过聚乙二醇（分子量6000）分级分离法，随后进行离子交换色谱法制备的，含有超过95%的完整IgG分子。一种特定的葡萄球菌免疫球蛋白是通过利凡诺/乙醇分级分离法从抗α毒素水平高的选定血清中获得的。当将这三种免疫球蛋白的作用确定为每克免疫球蛋白的活性（半数有效剂量）时，它们诱导的保护程度相似。