Leitão Diego A H S, Pedrosa Elvira M R, Dickson Donald W, Oliveira Ana Karina S, Rolim Mario Monteiro
Entomology and Nematology Department, University of Florida, Gainesville, FL, 32608.
Agricultural Engineering Department, Federal Rural University of Pernambuco, Recife, Pernambuco, 52171-900, Brazil.
J Nematol. 2021 Aug 12;53. doi: 10.21307/jofnem-2021-074. eCollection 2021.
The peach root-knot nematode, , is an emerging species and may become a threat to peach growers if contamination and spread are not avoided. The influence of temperature and two plants - tomato () and French marigold () - on the vertical migration of second-stage juveniles (J2) of was studied using 14-cm long segmented soil columns. Plants were transplanted into cups attached to the top of each column. Nylon meshes were placed between cups and columns to prevent downward root growth. About 1,000 freshly hatched J2 were injected into the base of the columns and then the columns were transferred to growth chambers at 20 and 26°C under a completely randomized block design with four replicates. The number of J2 in each ring of the columns as well as inside tomato or marigold roots was recorded at 3, 6, 9, and 12 days after injection (DAI). Nematode data were subjected to a repeated measures MANOVA. The presence of plants did not improve J2 migration as compared to control. migration was best at 20°C at first, with J2 migrating more than 13 cm as soon as 3 DAI, while it took 9 DAI for J2 to migrate long distances at 26°C. The distribution of J2 along the columns was similar at both temperatures at 12 DAI. Temperature had no influence on J2 penetration. French marigold did not hinder J2 migration, but fewer J2 penetrated its roots.
桃根结线虫是一个新出现的物种，如果不避免其污染和传播，可能会对桃种植者构成威胁。利用14厘米长的分段土柱，研究了温度以及番茄和法国万寿菊这两种植物对桃根结线虫二龄幼虫垂直迁移的影响。将植物移栽到附着在每个土柱顶部的杯子中。在杯子和土柱之间放置尼龙网，以防止根系向下生长。将约1000条刚孵化的二龄幼虫注射到土柱底部，然后将土柱转移到20℃和26℃的生长室中，采用完全随机区组设计，重复4次。在注射后3、6、9和12天记录土柱各环以及番茄或万寿菊根内二龄幼虫的数量。对线虫数据进行重复测量多变量方差分析。与对照相比，植物的存在并没有促进二龄幼虫的迁移。起初，二龄幼虫在20℃时迁移情况最佳，注射后3天二龄幼虫迁移距离超过13厘米，而在26℃时二龄幼虫需要9天才能远距离迁移。在注射后12天，两个温度下二龄幼虫沿土柱的分布相似。温度对二龄幼虫的侵入没有影响。法国万寿菊没有阻碍二龄幼虫的迁移，但侵入其根系的二龄幼虫较少。
