Univ. Lille, Inserm, CHU Lille, Laboratory of Development and Plasticity of the Postnatal Brain, Lille Neuroscience & Cognition, UMR-S1172, 59000 Lille, France.
STAR Protoc. 2021 Jul 31;2(3):100684. doi: 10.1016/j.xpro.2021.100684. eCollection 2021 Sep 17.
Here, we describe a protocol that provides the steps required for the generation of a mouse model of polycystic ovary syndrome (PCOS) by exposing dams to elevated levels of anti-Müllerian hormone during late gestation. This protocol also describes the steps required to assess the PCOS-like equivalents of the Rotterdam PCOS diagnostic criteria in mice. For complete details on the use and execution of this protocol, please refer to Tata et al. (2018) and Mimouni et al. (2021).
在这里，我们描述了一种通过在妊娠后期使母鼠暴露于高水平抗苗勒管激素来产生多囊卵巢综合征（PCOS）小鼠模型的方案。该方案还描述了评估小鼠中鹿特丹 PCOS 诊断标准的 PCOS 样等效物所需的步骤。有关该方案的使用和执行的完整详细信息，请参阅 Tata 等人。（2018 年）和 Mimouni 等人。（2021 年）。
