Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA.
Neuroscience Graduate Program, Mayo Clinic, Jacksonville, FL 32224, USA.
STAR Protoc. 2021 Aug 5;2(3):100715. doi: 10.1016/j.xpro.2021.100715. eCollection 2021 Sep 17.
Cerebral blood vessels supply oxygen and nutrients, remove metabolic waste, and play a critical role in maintaining brain homeostasis. Cerebrovasculature is composed of heterogeneous populations of brain vascular cells (BVCs). A major challenge in effective cerebrovascular transcriptional profiling is high-quality BVC procurement, permitting high sequencing depth. Here, we establish cell isolation procedures for glio-vascular cell-enriched single-cell RNA sequencing enabling unbiased characterization of BVC transcriptional heterogeneity. Our approach can be used to address vascular-specific contribution to brain diseases. For complete details on the use and execution of this protocol, please refer to Yamazaki et al. (2021).
脑血管供应氧气和营养物质，清除代谢废物，在维持脑内环境稳定方面起着至关重要的作用。脑血管系统由多种脑血管细胞（BVC）组成。进行有效的脑血管转录组分析的主要挑战是高质量的 BVC 分离，以实现高通量测序。在这里，我们建立了富含胶质-血管细胞的单细胞 RNA 测序的细胞分离程序，能够对 BVC 的转录异质性进行无偏特征分析。我们的方法可用于解决血管对脑部疾病的特异性影响。有关该方案使用和实施的完整详细信息，请参阅 Yamazaki 等人（2021 年）。
