Levin B E, Dunn-Meynell A, Sved A F
Neurology Service, Veterans Administration Medical Center, East Orange, NJ 07019.
Brain Res Bull. 1987 Dec;19(6):723-34. doi: 10.1016/0361-9230(87)90060-8.
The somatosensory area of fetal (E15-16) Sprague-Dawley rat neocortex was implanted into the barrel receptor area of adult first somatosensory cortex (SmI) to determine if it could be integrated into the host vibrissa-cortical pathway. Functional integration was tested 3-6 months later using the 2-deoxyglucose [44,45] (2-dg) method for estimation of glucose utilization. A variety of control grafts (cerebellum in SmI cortex, neocortex outside the host SmI cortex and appropriately placed neocortex in non-stimulated hosts) all had uniform 2-dg uptake (70 +/- 10 mumol/100 g/min) throughout each graft which averaged 42% less than non-stimulated host SmI cortex (121 +/- 6 mumol/100 g/min; p less than 0.001) but was 3-fold greater than white matter (24.8 +/- 3.1 mumol/100 g/min; p less than 0.001). In appropriately placed neocortical grafts, vibrissal stimulation produced 125% greater average 2-dg uptake (157 +/- 19 mumol/100 g/min) than control grafts (p less than 0.01). Such appropriately placed and stimulated neocortical grafts also contained focal areas of increased 2-dg uptake which were 43% greater than average graft uptake. These data suggest that fetal neocortical grafts were functionally integrated into the physiologically relevant afferent pathways of adult host brains.
将胎龄15 - 16天的Sprague-Dawley大鼠新皮质的体感区植入成年大鼠第一体感皮质（SmI）的桶状感受器区，以确定其能否整合到宿主触须 - 皮质通路中。3 - 6个月后，采用2 - 脱氧葡萄糖法[44,45]检测功能整合情况，该方法用于估计葡萄糖利用率。各种对照移植物（SmI皮质中的小脑、宿主SmI皮质外的新皮质以及置于未受刺激宿主中的适当位置的新皮质）在每个移植物中均具有均匀的2 - 脱氧葡萄糖摄取量（70±10 μmol/100 g/min），平均比未受刺激的宿主SmI皮质低42%（121±6 μmol/100 g/min；p<0.001），但比白质高3倍（24.8±3.1 μmol/100 g/min；p<0.001）。在适当位置的新皮质移植物中，触须刺激产生的平均2 - 脱氧葡萄糖摄取量（157±19 μmol/100 g/min）比对照移植物高125%（p<0.01）。这种适当位置且受刺激的新皮质移植物还包含2 - 脱氧葡萄糖摄取增加的局部区域，比移植物平均摄取量高43%。这些数据表明，胎儿新皮质移植物在功能上整合到了成年宿主大脑的生理相关传入通路中。
