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由链霉菌 ZL-24 产生的焦黑素及其对过氧化氢诱导的 SH-SY5Y 细胞损伤的保护作用。

Pyomelanin produced by Streptomyces sp. ZL-24 and its protective effects against SH-SY5Y cells injury induced by hydrogen peroxide.

机构信息

School of Bioscience and Biotechnology, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan, 250022, China.

School of Materials Science and Engineering, University of Jinan, Jinan, China.

出版信息

Sci Rep. 2021 Aug 17;11(1):16649. doi: 10.1038/s41598-021-94598-3.

DOI:10.1038/s41598-021-94598-3
PMID:34404820
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8371117/
Abstract

A soluble melanin pigment produced by Streptomyces sp. ZL-24 was purified and named StrSM. The elemental analysis of StrSM showed it consists of carbon, hydrogen, and oxygen. The spectrum analysis, including ultraviolet-visible absorption spectrum, Fourier-transform infrared spectrum, and pyrolysis-gas chromatography-mass spectrometry, indicated that StrSM might be pyomelanin. High performance liquid chromatography and liquid chromatography-mass spectra analysis of intermediate metabolite showed the presence of homogentisic acid (HGA). Moreover, the enzyme 4-hydroxyphenylpyruvate dioxygenase, involved in HGA biosynthesis, showed high activity during melanin production. Subsequently, a tyrosinase gene (melC2) and hydroxyphenylpyruvate dioxygenase gene double mutant demonstrated StrSM is pyomelanin. In vitro bioactivity assay showed that StrSM had excellent protective capability against SH-SY5Y cell oxidative injury. To our knowledge, the results firstly provide comprehensive data on Streptomyces pyomelanin identification and a promising candidate compound to treat oxidative injury of neurocytes.

摘要

一种由链霉菌 sp. ZL-24 产生的可溶性黑色素被纯化并命名为 StrSM。StrSM 的元素分析表明它由碳、氢和氧组成。光谱分析，包括紫外-可见吸收光谱、傅里叶变换红外光谱和热解气相色谱-质谱分析，表明 StrSM 可能是焦黑色素。中间代谢产物的高效液相色谱和液相色谱-质谱分析表明存在对羟基苯丙酮酸（HGA）。此外，参与 HGA 生物合成的酶 4-羟基苯丙酮酸双加氧酶在黑色素生成过程中表现出高活性。随后，酪氨酸酶基因（melC2）和 4-羟基苯丙酮酸双加氧酶基因双突变体表明 StrSM 是焦黑色素。体外生物活性测定表明，StrSM 对 SH-SY5Y 细胞氧化损伤具有极好的保护能力。据我们所知，这些结果首次提供了关于链霉菌焦黑色素鉴定的全面数据，以及一种有前途的治疗神经细胞氧化损伤的候选化合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/5020daf2e875/41598_2021_94598_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/140f9e4f984a/41598_2021_94598_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/87bf46f51dcc/41598_2021_94598_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/2d2f679ef355/41598_2021_94598_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/2c80e46e7643/41598_2021_94598_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/a7c82ead7daf/41598_2021_94598_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/5020daf2e875/41598_2021_94598_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/140f9e4f984a/41598_2021_94598_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/87bf46f51dcc/41598_2021_94598_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/2d2f679ef355/41598_2021_94598_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/2c80e46e7643/41598_2021_94598_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/a7c82ead7daf/41598_2021_94598_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4752/8371117/5020daf2e875/41598_2021_94598_Fig6_HTML.jpg

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