Nguyen Duong Thuy, Nguyen Vo Linh Phuong, Mangelings Debby, Van Eeckhaut Ann, Vander Heyden Yvan, Tran Hanh T M
Applied Microbiology Laboratory, School of Biotechnology, International University - VNU HCM, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam.
Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, Brussels, 1090, Belgium.
Sci Rep. 2025 Feb 19;15(1):6106. doi: 10.1038/s41598-025-90557-4.
Fungal melanins have remarkable bioactivities, but their practical applications are limited, partly due to their hydrophobic nature. Melanin modification with amino acids was reported to improve their water solubility. This research aimed to extract, and modify the melanins from Calvatia craniiformis and Xylaria plebeja with arginine, and to evaluate their neuroprotective effects against Parkinsonian toxins. MTT assay showed that all melanins and melanin derivatives could protect SH-SY5Y neuroblastoma cells from 1 mM H2O2. Xylaria plebeja melanin and melanin derivative at 100 µg/mL increased the cell viability about 16.0% compared to the negative control, whereas C. craniiformis melanin and its modified form enhanced the cell viability by 18.4% and 8.3%, respectively and that of the positive control (20 µM resveratrol) was 35.5%. Regarding neuroprotective effects against MPTP, the positive control, C. craniiformis melanin, and modified melanin increased the cell viability with approximately 5.0%, but that of X. plebeja melanin and modified melanin were 13.0%. Both melanins and melanin derivatives from C. craniiformis and X. plebeja showed significant neuroprotective effects against H2O2. However, only X. plebeja melanin and modified melanin displayed significant neuroprotective effects against MPTP and therefore they would be potential materials for follow-up research in Parkinson's disease prevention.
真菌黑色素具有显著的生物活性，但其实际应用受到限制，部分原因是其疏水性。据报道，用氨基酸修饰黑色素可提高其水溶性。本研究旨在从马勃菌和炭角菌中提取黑色素并用精氨酸对其进行修饰，并评估它们对帕金森毒素的神经保护作用。MTT 法显示，所有黑色素和黑色素衍生物都能保护 SH-SY5Y 神经母细胞瘤细胞免受 1 mM H2O2 的损伤。与阴性对照相比，100 µg/mL 的炭角菌黑色素和黑色素衍生物使细胞活力提高了约 16.0%，而马勃菌黑色素及其修饰形式分别使细胞活力提高了 18.4%和 8.3%，阳性对照（20 µM 白藜芦醇）为 35.5%。关于对 MPTP 的神经保护作用，阳性对照、马勃菌黑色素和修饰黑色素使细胞活力提高了约 5.0%，而炭角菌黑色素和修饰黑色素为 13.0%。马勃菌和炭角菌的黑色素及黑色素衍生物对 H2O2 均显示出显著的神经保护作用。然而，只有马勃菌黑色素和修饰黑色素对 MPTP 显示出显著的神经保护作用，因此它们将是帕金森病预防后续研究的潜在材料。
