Lundin S, Toresson G
Department of Clinical Pharmacology, Lund University Hospital, Sweden.
Horm Metab Res. 1987 Dec;19(12):629-31. doi: 10.1055/s-2007-1011896.
Immunoreactive arginine vasopressin (irAVP) was measured in seminal fluid with and without extraction using a specific radioimmunoassay (RIA). A large fraction of irAVP was removed after extraction on octadecasilylsilica cartridges. The measured amount of irAVP corresponded to the levels found in blood plasma. Dilutions of seminal plasma extracts were parallel with the RIA standard curve. On reversed phase HPLC the extracted material coeluted with synthetic AVP. These findings suggest an identity of this immunoreactive material with intact AVP. During incubations of synthetic AVP and its analogue 8-D-arginine vasopressing (8-DAVP) in seminal plasma, immunoreactivity decreased considerably with the former peptide, while the concentration of 8-DAVP was not significantly altered.
采用特异性放射免疫分析法（RIA）对有无提取的精液中的免疫反应性精氨酸加压素（irAVP）进行测定。在十八烷基硅烷键合硅胶柱上进行提取后，大部分irAVP被去除。测得的irAVP量与血浆中发现的水平相当。精液血浆提取物的稀释液与RIA标准曲线平行。在反相高效液相色谱法中，提取物与合成的AVP共洗脱。这些发现表明这种免疫反应性物质与完整的AVP相同。在精液血浆中合成AVP及其类似物8-D-精氨酸加压素（8-DAVP）的孵育过程中，前一种肽的免疫反应性显著降低，而8-DAVP的浓度没有明显改变。
