自体祖细胞和脱细胞猪动脉基质在组织工程血管移植物构建中的潜力。
Potential of Autologous Progenitor Cells and Decellularized Porcine Artery Matrix in Construction of Tissue-engineered Vascular Grafts.
机构信息
Departments Of Pediatrics, National Cheng Kung University Hospital, College Of Medicine, National Cheng Kung University, Tainan, Taiwan.
Departments Of Surgery, National Cheng Kung University Hospital, College Of Medicine, National Cheng Kung University, Tainan, Taiwan.
出版信息
Organogenesis. 2021 Oct 2;17(3-4):72-84. doi: 10.1080/15476278.2021.1963603. Epub 2021 Aug 18.
To develop a tissue-engineered vascular graft, we used pericardial effusion-derived progenitor cells (PEPCs) collected from drained fluid after open-heart surgery in children with congenital heart diseases to repopulate a decellularized porcine pulmonary artery. The PEPCs were compared with human fibroblasts (HS68) and human umbilical vein endothelial cells (HUVECs) in cell growth and migration. They were cultured with the matrices via an inner approach (intima), lateral approach (media), and outer approach (adventitia). PEPCs grew and migrated better than the other two cells 14 days after seeding in the decellularized vessel. In immunofluorescence assays, PEPCs expressed CD90 and CD105 indicating a vascular differentiation. PEPCs grew in a decellularized porcine pulmonary artery matrix may have the potential for producing tissue-engineered vascular grafts.
为了开发组织工程血管移植物，我们使用了从患有先天性心脏病的儿童开胸手术后引流的胸腔积液中分离出来的祖细胞（PEPCs），以填充脱细胞猪肺动脉。将 PEPCs 与人类成纤维细胞（HS68）和人脐静脉内皮细胞（HUVECs）在细胞生长和迁移方面进行了比较。通过内部途径（内膜）、侧面途径（中膜）和外部途径（外膜）将它们与基质一起培养。在脱细胞血管中接种 14 天后，PEPCs 的生长和迁移速度均优于其他两种细胞。在免疫荧光检测中，PEPCs 表达 CD90 和 CD105，表明其具有血管分化的潜能。PEPCs 可以在脱细胞猪肺动脉基质中生长，这可能为组织工程血管移植物的产生提供了潜力。
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