Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil.
Einstein (Sao Paulo). 2021 Aug 13;19:eAO5657. doi: 10.31744/einstein_journal/2021AO5657. eCollection 2021.
To evaluate the morphology and morphometry of the muscles extensor digitorium longus and soleus of C57BL/6 females, who were exposed to glyphosate during pregnancy and lactation.
Twelve female mice from the C57BL/6 lineage were used. After detection of pregnancy, they were divided into a Control Group, which received only water, and a Glyphosate Group, which received water with 0.5% glyphosate during pregnancy and lactation. Both groups received ad libitum standard diet. After weaning, the females were euthanized and weighed; naso-anal length was measured, and fats were collected and weighed. The muscles extensor digitorium longus and soleus were collected, and their length and weight were measured. Then, the muscles were fixed in Methacarn to perform the histological study of muscle fibers.
Glyphosate Group presented lower weight gain during pregnancy and also lower final body weight and naso-anal length; however, the other body parameters evaluated did not present a significant difference in relation to the Control Group. Significant differences were also not observed in the analysis of muscle fibers and connective tissue.
Exposure to 0.5% glyphosate during pregnancy and lactation resulted in lower weight gain during pregnancy, final weight, and naso-anal length. Despite not directly altering the morphology of muscle tissue, these results may indicate enough exposure to interfere with animal metabolism.
评估暴露于孕期和哺乳期草甘膦的 C57BL/6 雌性个体的趾长伸肌和比目鱼肌的形态和形态计量学。
使用 12 只来自 C57BL/6 品系的雌性小鼠。妊娠检测后，它们被分为对照组（仅接受水）和草甘膦组（孕期和哺乳期接受含 0.5%草甘膦的水）。两组均接受自由标准饮食。断奶后，雌性动物被安乐死并称重；测量鼻肛长度；收集并称重脂肪。收集趾长伸肌和比目鱼肌，测量其长度和重量。然后，将肌肉固定在 Methacarn 中，进行肌肉纤维的组织学研究。
草甘膦组在孕期的体重增加较低，最终体重和鼻肛长度也较低；然而，与对照组相比，其他评估的身体参数没有显著差异。肌肉纤维和结缔组织的分析也没有观察到显著差异。
孕期和哺乳期暴露于 0.5%草甘膦导致孕期体重增加、最终体重和鼻肛长度降低。尽管没有直接改变肌肉组织的形态，但这些结果可能表明足够的暴露会干扰动物的新陈代谢。
