Vincek Erik, Rudnick Eric
Frank H. Netter MD School of Medicine, North Haven, CT, USA.
Department of Dermatology, College of Medicine, University of Florida, Gainesville, NW, USA.
J Histotechnol. 2022 Mar;45(1):36-38. doi: 10.1080/01478885.2021.1964872. Epub 2021 Aug 19.
Melan-A is one of the most commonly used immunohistochemical assays (IHC) in dermatopathology laboratories to detect the presence and outline the distribution of melanocytes. It is a cytoplasmic stain that detects a melanocyte-specific cytoplasmic protein involved in the formation of stage II melanosomes. Clinically, Melan-A is primarily used to detect and confirm melanocytic tumors although it is also positively expressed in adrenal cortical tumors and sex cord stromal tumors. We found that Melan-A also detected and highlighted Henderson-Patterson bodies of molluscum poxvirus. To determine if other melanocytic markers detect molluscum contagiosum bodies, S-100, HMB-45, MITF, and SOX-10 were also tested. In 15 tested molluscum cases, Melan-A stains were positive in all cases, whereas the other tested melanocytic markers were negative. Our results confirm that Melan-A is very sensitive in detecting molluscum contagiosum bodies and could be clinically useful to supplement the hematoxylin and eosin (H&E) in cases that are very inflamed or only have limited biopsy material.
Melan-A是皮肤病理学实验室中最常用的免疫组织化学检测方法（IHC）之一，用于检测黑素细胞的存在并勾勒其分布。它是一种细胞质染色剂，可检测参与II期黑素小体形成的黑素细胞特异性细胞质蛋白。临床上，Melan-A主要用于检测和确诊黑素细胞肿瘤，尽管它在肾上腺皮质肿瘤和性索间质肿瘤中也呈阳性表达。我们发现Melan-A还能检测并突出显示痘病毒软疣的亨德森-帕特森小体。为了确定其他黑素细胞标志物是否能检测传染性软疣小体，我们还检测了S-100、HMB-45、MITF和SOX-10。在15例检测的传染性软疣病例中，所有病例的Melan-A染色均为阳性，而其他检测的黑素细胞标志物均为阴性。我们的结果证实，Melan-A在检测传染性软疣小体方面非常敏感，在炎症非常严重或活检材料有限的病例中，对补充苏木精和伊红（H&E）染色具有临床应用价值。
