Pathogen Biology and Immunology Laboratory and Laboratory of Tissue and Cell Biology, Experimental Teaching and Management Center, Chongqing Medical University, Chongqing 401331, China.
Department of the First Clinical Medicine, Chongqing Medical University, Chongqing 401331, China.
Biomed Res Int. 2021 Aug 9;2021:1807293. doi: 10.1155/2021/1807293. eCollection 2021.
Human Parainfluenza Virus Type 3 (HPIV3) is one of the main pathogens that cause acute lower respiratory tract infections in infants and young children. However, there are currently no effective antiviral drugs and vaccines. Herein, we found that a natural compound, curcumin, inhibits HPIV3 infection and has antiviral effects on entry and replication of the virus life cycle. Immunofluorescence and western blotting experiments revealed that curcumin disrupts F-actin and inhibits viral inclusion body (IB) formation, thus inhibiting virus replication. Curcumin can also downregulate cellular PI4KB and interrupt its colocalization in viral IBs. This study verified the antiviral ability of curcumin on HPIV3 infection and preliminarily elucidated its influence on viral replication, providing a theoretical basis for antiviral drug development of HPIV3 and other parainfluenza viruses.
人副流感病毒 3 型（HPIV3）是引起婴幼儿急性下呼吸道感染的主要病原体之一。然而，目前尚无有效的抗病毒药物和疫苗。在这里，我们发现一种天然化合物姜黄素抑制 HPIV3 感染，并对病毒生命周期的进入和复制具有抗病毒作用。免疫荧光和 Western blot 实验表明，姜黄素破坏 F-actin 并抑制病毒包涵体（IB）的形成，从而抑制病毒复制。姜黄素还可以下调细胞 PI4KB 并中断其在病毒 IB 中的共定位。本研究验证了姜黄素对 HPIV3 感染的抗病毒能力，并初步阐明了其对病毒复制的影响，为开发 HPIV3 和其他副流感病毒的抗病毒药物提供了理论依据。
