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内毒素上存在2-羟基肉豆蔻酸盐与患有复杂性脓毒性休克的新生儿死亡有关。

Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with complex septic shock.

作者信息

Augusto Luis A, Bourgeois-Nicolaos Nadège, Breton Aude, Barreault Simon, Alonso Enrique Hernandez, Gera Stuti, Faraut-Derouin Véronique, Semaan Nada, De Luca Daniele, Chaby Richard, Doucet-Populaire Florence, Tissières Pierre

机构信息

Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, France.

Department of Bacteriology-Hygiene, AP-HP Paris-Saclay, Hôpital Antoine Béclère, Clamart Cedex, France.

出版信息

iScience. 2021 Jul 30;24(8):102916. doi: 10.1016/j.isci.2021.102916. eCollection 2021 Aug 20.

DOI:10.1016/j.isci.2021.102916
PMID:34409274
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8361193/
Abstract

complex species are involved in infections among critically ill patients. After a recent outbreak of fulminant neonatal septic shock, we conducted a study to determine whether septic shock severity and its lethal consequence are related to structural features of the endotoxin (lipopolysaccharide [LPS]) of the strains isolated from hospitalized infants and more specifically its lipid A region. It appeared that the LPSs are very heterogeneous, carrying fifteen different molecular species of lipid A. The virulence was correlated with a structural feature identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry and gas chromatography coupled with mass spectrometry: the presence of 2-hydroxymyristic acid as a secondary substituent in lipid A. This is the first published evidence linking LPS structural moiety to neonatal sepsis outcome and opens the possibility of using this fatty acid marker as a detection tool for high-risk patients, which could help reduce their mortality.

摘要

复杂的细菌种类参与了重症患者的感染。在最近一次暴发性新生儿脓毒性休克疫情之后，我们开展了一项研究，以确定脓毒性休克的严重程度及其致命后果是否与从住院婴儿中分离出的菌株的内毒素（脂多糖 [LPS]）的结构特征有关，更具体地说是与它的脂质A区域有关。结果显示，LPS非常异质，带有15种不同分子种类的脂质A。毒力与通过基质辅助激光解吸电离飞行时间质谱和气相色谱-质谱联用鉴定的一种结构特征相关：脂质A中存在2-羟基肉豆蔻酸作为二级取代基。这是首次发表的将LPS结构部分与新生儿败血症结局联系起来的证据，并开启了将这种脂肪酸标志物用作高危患者检测工具的可能性，这有助于降低他们的死亡率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/953c94cd169f/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/14c912ae32a6/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/081a7bb82fa6/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/9c0292782d90/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/7b85a7e83954/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/e6755ce9ffb0/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/afc898ddab8d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/392f11782da1/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/a21dc67a7347/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/9deeaa4e77d6/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/953c94cd169f/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/14c912ae32a6/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/081a7bb82fa6/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/9c0292782d90/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/7b85a7e83954/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/e6755ce9ffb0/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/afc898ddab8d/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/392f11782da1/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/a21dc67a7347/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/9deeaa4e77d6/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52a4/8361193/953c94cd169f/gr9.jpg

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