Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei, Anhui, China.
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Hefei, Anhui, China,
Avian Dis. 2021 Jun;65(2):237-240. doi: 10.1637/aviandiseases-D-21-00020.
is a trichomonad protozoan parasite that can cause an important poultry disease known as histomoniasis; Marek's disease virus (MDV) and subtype J avian leukosis virus (ALV-J) usually cause avian oncogenic diseases. Although these diseases have been reported in a single pathogen infection, information about their coinfection is scarce. This study reports a naturally occurring case of coinfection with , MDV, and ALV-J in a local chicken flock at the age of 150 days. Necropsy revealed necrosis and swelling in the liver and spleen. Histologic analysis showed large areas of mild to severe necrosis of hepatocytes, with numerous intralesional trophozoites of by H&E and periodic acid-Schiff staining; H&E staining showed pleomorphic and neoplastic lymphoid tumor cells in the liver and myeloid cells with eosinophilic cytoplasmic granules in the spleen. Coexpression of MDV and ALV-J antigens was detected in the liver by fluorescence multiplex immunohistochemistry staining. The gene of , gene of MDV, and gene of ALV-J were identified in mixed liver and spleen tissues by PCR and sequencing, respectively.
是一种纤毛虫原生动物寄生虫，可引起一种名为组织滴虫病的重要家禽疾病；马立克氏病病毒（MDV）和 J 亚群禽白血病病毒（ALV-J）通常引起禽类致癌疾病。尽管这些疾病已在单一病原体感染中报道，但有关其合并感染的信息却很少。本研究报告了一例在 150 日龄本地鸡群中发生的 、MDV 和 ALV-J 自然合并感染的病例。剖检显示肝脏和脾脏坏死和肿胀。组织学分析显示，肝细胞有大面积的轻度至重度坏死，通过 H&E 和过碘酸雪夫染色可见大量的组织内滋养体；H&E 染色显示肝脏中有异型和肿瘤性淋巴样肿瘤细胞，脾脏中有嗜酸性细胞质颗粒的髓样细胞。通过荧光多重免疫组织化学染色在肝脏中检测到 MDV 和 ALV-J 抗原的共表达。通过 PCR 和测序分别从混合的肝脏和脾脏组织中鉴定出 、MDV 的 基因和 ALV-J 的 基因。
