Epidemiol Mikrobiol Imunol. 2021 Summer;70(2):110-117.
To present the results of whole genome sequencing (WGS) analysis of Streptococcus pneumoniae serotype 19A and to compare them with the respective data from Europe. The vaccine serotype 19A is widely distributed in the Czech Republic.
WGS was used in this study as the most powerful available method for detailed characterization of S. pneumoniae. Nineteen Czech isolates of S. pneumoniae 19A were analysed and compared with 415 European isolates included in the PubMLST database.
S. pneumoniae serotype 19A causes all types of pathogen - host interaction, from carriage to noninvasive and invasive pneumococcal disease (IPD). In 2010 - 2019, 3872 cases of IPD were reported within the surveillance programme in the Czech Republic, with 323 of these caused by serotype 19A. WGS data of the Czech serotype 19A isolates show a numerous and genetically related subpopulation of three sequencing types: ST-199, ST-416, and ST-3017. Within this subpopulation, the largest is the cluster of nine ST-199 isolates. High relatedness of ST-199 isolates is also confirmed by the fact that all but one isolate, 117/2019 (novel rST- -137805), share the same ribosome sequencing profile - rST-11365. Outside the above-mentioned subpopulation, there are only four isolates that form three separate genetic lines of serotype 19A. A highly similar situation is observed across European countries, where about half of all serotype 19A isolates form a genetically closely related subpopulation (ST-199, ST-416, ST-450, ST-667, ST-3017, and ST-10360) while isolates which are not part of this subpopulation represent a large number of unrelated genetic lines.
The study has shown a mostly homogeneous population of S. pneumoniae serotype 19A to circulate in the post-vaccination era in both the Czech Republic and Europe, with some unrelated isolates located outside this population.
介绍肺炎链球菌 19A 血清型全基因组测序（WGS）分析结果，并与欧洲相应数据进行比较。疫苗血清型 19A 在捷克共和国广泛分布。
本研究采用 WGS 作为最强大的方法，用于详细描述肺炎链球菌。分析了 19 株捷克肺炎链球菌 19A 分离株，并与 PubMLST 数据库中包含的 415 株欧洲分离株进行比较。
肺炎链球菌血清型 19A 引起从带菌到非侵袭性和侵袭性肺炎球菌病（IPD）的所有类型的病原体-宿主相互作用。在 2010-2019 年期间，捷克共和国的监测计划报告了 3872 例 IPD 病例，其中 323 例由血清型 19A 引起。捷克血清型 19A 分离株的 WGS 数据显示了三个测序型的大量且遗传相关的亚群：ST-199、ST-416 和 ST-3017。在这个亚群中，最大的是 9 株 ST-199 分离株的聚类。ST-199 分离株的高度相关性也得到证实，因为除了一个分离株 117/2019（新型 rST- -137805）之外，所有分离株都具有相同的核糖体测序谱-rST-11365。在上述亚群之外，只有四个分离株形成了三个独立的 19A 血清型遗传谱系。在欧洲各国也观察到非常相似的情况，其中约一半的血清型 19A 分离株形成了遗传上密切相关的亚群（ST-199、ST-416、ST-450、ST-667、ST-3017 和 ST-10360），而不属于该亚群的分离株则代表了大量无关的遗传谱系。
该研究表明，在捷克共和国和欧洲，肺炎链球菌 19A 血清型在接种疫苗后时代主要是一个同质的流行人群，而一些无关的分离株则位于该人群之外。
