Kozáková J, Vohrnová S, Honskus M, Křížová P
Epidemiol Mikrobiol Imunol. 2024;73(2):84-97. doi: 10.61568/emi/11-6306/20240424/137081.
An analysis is presented of whole genome data of Streptococcus pneumoniae serotypes 8 and 22F isolated in the Czech Republic from invasive pneumococcal disease (IPD) in 2014-2020. New multivalent pneumococcal conjugate vaccines (PCVs) are effective against these serotypes. Recently, serotypes 8 and 22F have been among the leading causes of IPD in the Czech Republic. S. pneumoniae isolates from the Czech Republic were compared with those of the same serotypes recovered in other countries in the same period and available in the international database PubMLST.
Isolates from IPD of serotypes 8 (22 isolates) and 22F (21 isolates) recovered in the Czech Republic in 2014-2020 were subjected to whole genome sequencing (WGS). The genomes were analysed and compared using the international database PubMLST.
Most of the studied Czech serotype 8 isolates belong to two main subpopulations. The first subpopulation, dominated by ST-53 isolates, is part of a highly abundant group of genetically close European and non-European isolates that are clearly separated on the phylogenetic network. The second subpopulation of Czech serotype 8 isolates (dominated by ST-404) is more genetically variable and forms a separate lineage on the global phylogenetic network, with no other European isolates. Czech isolates of serotype 22F are a homogeneous population with a clear predominance of ST-433, which belongs to a genetically close European population.
The analysis of WGS data of IPD isolates of serotypes 8 and 22F provided a detailed insight into the genetic relationships between the Czech populations of these serotypes. It also allowed comparison of the Czech populations with the matched populations from other European and non-European countries. The obtained results add to the body of knowledge about the spread of genetic lineages causing IPD in the Czech Republic in the post-vaccination period and provide a basis for considering whether the use of the new multivalent PCVs in the Czech Republic would be beneficial.
对2014年至2020年在捷克共和国从侵袭性肺炎球菌疾病（IPD）中分离出的8型和22F型肺炎链球菌的全基因组数据进行分析。新型多价肺炎球菌结合疫苗（PCV）对这些血清型有效。最近，8型和22F型血清型已成为捷克共和国IPD的主要病因之一。将来自捷克共和国的肺炎链球菌分离株与同期在其他国家回收并可在国际数据库PubMLST中获得的相同血清型的分离株进行比较。
对2014年至2020年在捷克共和国从IPD中分离出的8型（22株）和22F型（21株）血清型分离株进行全基因组测序（WGS）。使用国际数据库PubMLST对基因组进行分析和比较。
大多数研究的捷克8型血清型分离株属于两个主要亚群。第一个亚群以ST-53分离株为主，是一组在系统发育网络上明显分开的、在欧洲和非欧洲高度丰富的遗传密切相关分离株的一部分。捷克8型血清型分离株的第二个亚群（以ST-404为主）遗传变异性更大，在全球系统发育网络上形成一个单独的谱系，没有其他欧洲分离株。捷克22F型血清型分离株是一个同质群体，ST-433明显占优势，ST-433属于遗传密切相关的欧洲群体。
对8型和22F型血清型IPD分离株的WGS数据进行分析，深入了解了这些血清型在捷克群体之间的遗传关系。它还允许将捷克群体与来自其他欧洲和非欧洲国家的匹配群体进行比较。所获得的结果增加了关于疫苗接种后时期在捷克共和国导致IPD的遗传谱系传播的知识体系，并为考虑在捷克共和国使用新型多价PCV是否有益提供了依据。
