环状RNA的特征分析
Characterization of Circular RNAs.
作者信息
Zhang Yang, Yang Li, Chen Ling-Ling
机构信息
Department of Medicine and Pathology, Cancer Research Institute, Beth Israel Deaconess Cancer Center, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
出版信息
Methods Mol Biol. 2021;2372:179-192. doi: 10.1007/978-1-0716-1697-0_16.
Accumulated lines of evidence have revealed that a large number of circular RNAs are produced in transcriptomes from fruit fly to mouse and human. Unlike linear RNAs shaped with 5' cap and 3' tail, circular RNAs are characterized by covalently closed loop structures without open terminals, thus required specific treatments for their identification and validation. Here, we describe a detailed pipeline for the characterization of circular RNAs. It has been successfully applied to the study of circular intronic RNAs (ciRNAs) derived from intron lariats and circular RNAs (circRNAs) produced from back spliced exons in human.
越来越多的证据表明，从果蝇到小鼠和人类的转录组中都会产生大量的环状RNA。与具有5'帽和3'尾结构的线性RNA不同，环状RNA的特征是具有共价闭合的环状结构，没有开放末端，因此需要特定的处理方法来进行鉴定和验证。在这里，我们描述了一个用于环状RNA表征的详细流程。它已成功应用于人类中源自内含子套索的环状内含子RNA（ciRNA）和反向剪接外显子产生的环状RNA（circRNA）的研究。
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