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The detection of human B lymphocytes by both light and electron microscopy utilizing colloidal gold labeled anti-immunoglobulin.

作者信息

Geoghegan W D, Scillian J J, Ackerman G A

出版信息

Immunol Commun. 1978;7(1):1-12. doi: 10.3109/08820137809033919.

DOI:10.3109/08820137809033919
PMID:344196
Abstract

Colloidal gold was used to label rabbit anti-goat IgG for the indirect detection of goat anti-human IgD and IgM. Utilizing this technique, surface immunoglobulin was visualized on human blood B lymphocytes at both the light and electron microscopic levels of resolution. By this technique, percentages of lymphocytes were found to closely correlate with those of the standard immunofluorescent procedure. The ease of the procedure along with the clarity of the marker in both light and electron microscopy suggest wide applicability in clinical and research studies of lymphocyte membrane markers.

摘要

相似文献

1
The detection of human B lymphocytes by both light and electron microscopy utilizing colloidal gold labeled anti-immunoglobulin.
Immunol Commun. 1978;7(1):1-12. doi: 10.3109/08820137809033919.
2
IgM- and IgD-bearing peripheral blood lymphocytes differentiate to IgM but not IgG or IgA immunoglobulin-secreting cells.携带IgM和IgD的外周血淋巴细胞分化为分泌IgM而非IgG或IgA免疫球蛋白的细胞。
Eur J Immunol. 1982 Jun;12(6):506-10. doi: 10.1002/eji.1830120611.
3
Colloidal gold as a marker for the light microscope detection of leukocyte cell surface antigens with monoclonal antibodies.胶体金作为用单克隆抗体进行白细胞细胞表面抗原光镜检测的标记物。
J Clin Immunol. 1982 Jul;2(3 Suppl):24S-31S.
4
Enumeration of immunoglobulin-bearing lymphocytes in whole peripheral blood.全外周血中携带免疫球蛋白淋巴细胞的计数
Clin Exp Immunol. 1976 Oct;26(1):91-4.
5
Occurrence of surface IgM, IgD, and free light chains of human lymphocytes.人类淋巴细胞表面免疫球蛋白M、免疫球蛋白D和游离轻链的出现情况。
J Exp Med. 1974 Feb 1;139(2):451-6. doi: 10.1084/jem.139.2.451.
6
Failure of B lymphocytes in human blood to regenerate surface immunoglobulin after its removal by antibody.人体血液中的B淋巴细胞在其表面免疫球蛋白被抗体清除后无法再生。
J Immunol. 1977 Jul;119(1):327-9.
7
The use of anti-immunoglobulins to induce a signal for cell division in B lymphocytes via their membrane IgM and IgD.使用抗免疫球蛋白通过B淋巴细胞的膜IgM和IgD诱导细胞分裂信号。
Immunol Rev. 1980;52:181-210. doi: 10.1111/j.1600-065x.1980.tb00335.x.
8
Allotypes of IgM and IgD receptors in the mouse: a probe for lymphocyte differentiation.小鼠中IgM和IgD受体的同种异型：淋巴细胞分化的一种探针。
Contemp Top Immunobiol. 1978;8:203-43. doi: 10.1007/978-1-4684-0922-2_7.
9
Anti-IgM but not anti-IgD antibodies inhibit cell division of normal human mature B cells.抗IgM抗体而非抗IgD抗体可抑制正常人成熟B细胞的细胞分裂。
J Immunol. 1992 Jan 1;148(1):29-34.
10
F(ab')2 reagents are not required if goat, rather than rabbit, antibodies are used to detect human surface immunoglobulin.如果使用山羊抗体而非兔抗体来检测人表面免疫球蛋白，则不需要F(ab')2试剂。
J Immunol. 1977 Sep;119(3):1084-8.

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Histochemistry. 1984;81(6):603-8. doi: 10.1007/BF00489542.
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Light microscopical detection of leukocyte cell surface antigens with a one-nanometer gold probe.用一纳米金探针进行白细胞细胞表面抗原的光学显微镜检测。
Histochemistry. 1991;95(5):483-90. doi: 10.1007/BF00315744.
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New sensitive light microscopical detection of colloidal gold on ultrathin sections by reflection contrast microscopy. Combination of reflection contrast and electron microscopy in post-embedding immunogold histochemistry.通过反射对比显微镜在超薄切片上对胶体金进行新的灵敏光学显微镜检测。包埋后免疫金组织化学中反射对比与电子显微镜的结合。
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