人类多形核白细胞的定量吞噬作用。使用放射性标记乳剂测量吞噬作用速率。
Quantitative phagocytosis by human polymorphonuclear leucocytes. Use of radiolabelled emulsions to measure thae rate of phagocytosis.
作者信息
Forsgren A, Schmeling D, Zettervall O
出版信息
Immunology. 1977 Apr;32(4):491-7.
Abstract
A new micro-method for the quantitative measurement of phagocytosis by neutrophils is described. The material used for phagocytosis consists of a radioactive oil emulsion coated with E. coli lipopolysaccharide. Uptake of radioactive material is a function of cell number, duration of incubation, dilution of serum used for opsonization, content of lipopolysaccharide and concentration of emulsion. This method can be used to quantify rapidly and precisely phagocytosis rates of as few as 5 x 10(4)-10(6) polymorphonuclear leucocytes and the opsonic activity of 10 microliter serum.
摘要
本文描述了一种用于定量测量中性粒细胞吞噬作用的新微方法。用于吞噬作用的物质是一种涂有大肠杆菌脂多糖的放射性油乳剂。放射性物质的摄取是细胞数量、孵育时间、用于调理素化的血清稀释度、脂多糖含量和乳剂浓度的函数。该方法可快速、精确地量化低至5×10⁴ - 10⁶个多形核白细胞的吞噬率以及10微升血清的调理活性。
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Studies on phagocytosis. I. Uptake of radio-iodinated (131-I) human serum albumin as a measure of the degree of phagocytosis in vitro.吞噬作用的研究。I. 采用放射性碘化（131-I）人血清白蛋白的摄取量作为体外吞噬作用程度的一种衡量指标。
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The human alveolar macrophage: isolation, cultivation in vitro, and studies of morphologic and functional characteristics.人肺泡巨噬细胞：分离、体外培养以及形态学和功能特性研究。
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