Pereira H A, Shelton M J, Hosking C S
J Clin Lab Immunol. 1983 May;11(1):47-53.
A neutrophil iodination micro-method has been developed for assessment of neutrophil and opsonic function in any serum/micro-organism combination. The test is performed in micro-titre trays and thus requires relatively small amounts of reagents and numbers of cells. The variables of the test have been explored and the effect of immunization with different organisms on opsonin levels defined. The versatility of the test has been demonstrated in its ability to measure both heat labile and heat stable opsonins in normal as well as immune serum, for a number of different micro-organisms. The method is simple, rapid and a large number of tests can be performed at the one time.
已开发出一种中性粒细胞碘化微量法，用于评估任何血清/微生物组合中的中性粒细胞和调理素功能。该测试在微量滴定板中进行，因此所需试剂和细胞数量相对较少。已对该测试的变量进行了探索，并确定了用不同生物体免疫对调理素水平的影响。该测试的多功能性已通过其在正常和免疫血清中测量多种不同微生物的热不稳定和热稳定调理素的能力得到证明。该方法简单、快速，并且可以同时进行大量测试。
