Locke Rebecca K, Greig David R, Jenkins Claire, Dallman Tim J, Cowley Lauren A
University of Bath, Claverton Down Campus, Bath, UK.
Gastrointestinal Reference Services, Public Health England, London, UK.
Microb Genom. 2021 Aug;7(8). doi: 10.1099/mgen.0.000644.
Shigellosis in men who have sex with men (MSM) is caused by multidrug resistant Shigellae, exhibiting resistance to antimicrobials including azithromycin, ciprofloxacin and more recently the third-generation cephalosporins. We sequenced four -positive MSM isolates (2018-20) using Oxford Nanopore Technologies; three (identified as two MSM clade 2, one MSM clade 5) and one 3a, to explore AMR context. All isolates harboured Tn7/Int2 chromosomal integrons, whereas 3a contained the Resistance Locus. All strains harboured IncFII pKSR100-like plasmids (67-83kbp); where present was located on these plasmids flanked by IS and IS, however was lost in 3a during storage between Illumina and Nanopore sequencing. IncFII AMR regions were mosaic and likely reorganised by IS; three of the four plasmids contained azithromycin-resistance genes ) and ) and one harboured the pKSR100 integron. Additionally, all isolates possessed a large IncB/O/K/Z plasmid, two of which carried and ). Monitoring the transmission of mobile genetic elements with co-located AMR determinants is necessary to inform empirical treatment guidance and clinical management of MSM-associated shigellosis.
男男性行为者（MSM）中的志贺菌病由多重耐药志贺菌引起，这些志贺菌对包括阿奇霉素、环丙沙星以及最近的第三代头孢菌素在内的抗菌药物均表现出耐药性。我们使用牛津纳米孔技术对4株阳性MSM分离株（2018 - 20年）进行了测序；其中3株（鉴定为2株MSM进化枝2、1株MSM进化枝5）和1株3a，以探究抗菌药物耐药性（AMR）情况。所有分离株都含有Tn7/Int2染色体整合子，而3a含有耐药基因座。所有菌株都含有IncFII pKSR100样质粒（67 - 83kbp）；在存在的情况下，位于这些质粒上，两侧为IS和IS，然而在Illumina和纳米孔测序之间的储存过程中，3a中的该质粒丢失了。IncFII AMR区域是镶嵌的，可能由IS进行了重组；4个质粒中的3个含有阿奇霉素耐药基因（）和），1个含有pKSR100整合子。此外，所有分离株都拥有一个大的IncB/O/K/Z质粒，其中两个携带和）。监测带有共定位AMR决定簇的移动遗传元件的传播对于为MSM相关志贺菌病的经验性治疗指导和临床管理提供信息是必要的。
